Oct-1-ene-3-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

The study was conducted between 15 August 2017 and 17 August 2017 (biological phase).

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Oct-1-ene-3-ol

EC Number:

222-226-0

EC Name:

Oct-1-ene-3-ol

Cas Number:

3391-86-4

Molecular formula:

C8H16O

IUPAC Name:

oct-1-en-3-ol

Test material form:

liquid

Specific details on test material used for the study:

Batch No.: VE00440179
Colour and Appearance: colourless to pale yellow liquid
Purity: 99.6%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples of stock solution and test solutions were taken to determine the actual test item concentrations in comparison to the nominally applied concentrations. Samples of test solutions were taken at the start of exposure in the fresh test solution (0 hour) and in the aged test solution (48h)

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution (S1) of the test item was prepared by dissolving 0.1003 g of the test item in 1000 mL of test medium, resulting in a nominal concentration of 100 mg/L. This stock solution was stirred for 1-h at ambient temperature in the dark. Subsequent inspection of the solution showed no undissolved matter. The stock solution was used immediately to prepare the desired test item concentrations. The stock solution was used, undiluted, as the highest test concentration. The diluted test solutions were stirred for another 10 min.

Frequency and Duration of Administration, Treatment Levels, and Replication
Based on the results of a preliminary non-GLP range finding test, the following concentrations in a geometrical series (spacing factor: 1.77) were tested in the definitive test: 10.2, 18.0, 31.9, 56.5 and 100 mg test item/L. The test period (exposure of test organisms to the test solutions in a static system) was 48 hours. Additionally, daphnids were exposed under control conditions (non-treated test medium without solvent).
Four replicates were used for the test item concentrations and the control. Each replicate vessel contained five daphnids.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Test System

• Species: The test system used in this study was Daphnia magna STRAUS (clone M10), a water flea, cultured at ECT Oekotoxikologie GmbH since December 22, 2011.
• Origin: The organisms were originally supplied by KU Leuven, Belgium.

The health of the Daphnia culture and the overall quality of the test organisms is monitored regularly by using the reference toxicant potassium dichromate (K2Cr2O7) in an acute test design in order to determine the sensitivity of the organisms from the culture and to demonstrate that the test conditions are appropriate.

The most recent reference test with potassium dichromate performed in a separate study (Study No. IDA1705) resulted in an EC50(24 h) of 1.29 mg/L.

According to the OECD guideline No. 202 (OECD, April 2004) EC50(24 h)-values for potassium dichromate obtained from different laboratories should range between 0.6 mg/l and 2.1 mg/l. The toxicity of the reference item is within this range. Therefore the results of this reference test are acceptable and the test conditions are reliable and the sensitivity of the test system could be demonstrated.

Study design

Test type:

static

Water media type:

other: Elendt medium M4 was used to dilute the test item and to keep the daphnids during the period of the test.

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19.7 - 20.9 °C

pH:

7.7 - 7.8

Nominal and measured concentrations:

Nominal: 10.2, 18.0, 31.9, 56.5 and 100 mg test item/L.
Geometric mean: 3.67, 8.61, 24.04, 43.26, 48.72 mg test item/L.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

8.02 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CI: 6.27 to 10.29 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

other: EC20

Effect conc.:

5.13 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

4.07 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Biological results: The % immobility reflected the concentration series. At 48h the percentage of immobilised daphnids was 0% (control), 10% (10.2mg/L nominal), 50% (18mg/L nominal) and 100% (31.9, 56.5 and 100mg/L nominal). Sublethal observations on the mobile daphnids were recorded. At 10.2, 18.0, 31.9 and 56.5 mg/L after 24 h and at 10.2 and 18.0 mg/L after 48 h, an increasing number of floaters and reduced swimming activity were observed.

Analytical Results: The measured concentrations in the test solutions at the start of exposure (0h) ranged from 25 to 83% of the nominal concentration and at the end of exposure (48h) from 34-84% of nominal. Given that the measured concentrations were not within 80% of the nominal concentrations, the biological endpoints reported are based on the geometric mean measured concentrations. The latter were calculated to be 3.67, 8.81, 24.04, 43.26 and 48.72 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the statistical evaluation of biological results and geometric measured concentrations of the test item, the 48-hour EC50 for Daphnia magna was 8.02 mg/L (95% confidence limits: 6.27 - 10.29 mg/L).

Executive summary:

The acute toxicity of the test item Amyl Vinyl Carbinol to Daphnia magna was determined in a 48-hour test according to the OECD Guideline for Testing of Chemicals, No. 202 (2004) and the Commission Regulation (EC) No. 440/2008, Part C.2.

Based on the statistical evaluation of biological results and geometric measured concentrations of the test item, the 48-hour EC50 for Daphnia magna was 8.02 mg/L (95% confidence limits: 6.27 - 10.29 mg/L).