Lubricating oils, used, distd.

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: OECD 23:2019

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

not specified

Details on sampling:

The sample is not soluble in water. A stock Water Accommodated Fraction (WAF) was prepared, then diluted to get the other tested doses. This is acceptable according to point 149 of § 7.9.2.4 “Preparing water-accommodated fractions (WAFs)” of the guidelines OECD 23:2019 – “Guidance document on aquatic toxicity testing of difficult substances and mixtures”. In fact, in the present study it was not be possible to prepare each WAF individually. This was due to the limitations of weighing small amounts of UVCB and the large test solution volumes required. An ASTM Type III water was used for elution and dilutions. The test medium was prepared adding the above salts to the WAF and its dilutions

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20°C

pH:

ca.8

Details on test conditions:

Test concentrations were prepared from a stock WAF at a loading rate (nominal concentration) of 100 mg/L. Tested doses are : 1 mg/L, 10 mg/L, 25 mg/L, 50 mg/L, 75 mg/L and 100 mg/L.

Reference substance (positive control):

yes

Key result

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: WAF

Details on results:

At test concentrations, the test sample induces negligible negative effects on crustaceans. The number of immobilized specimens never exceeds 30% for each tested concentration and is not proportional to the dose. At the maximum tested nominal concentration of 100 mg/L, the WAF immobilizes only 15% of the specimens. Thus, we can consider that the value of EL50 is greater than any tested concentration, and EL50 > 100 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

The Daphnia magna EL50 is greater than 100 mg/L, which is the maximum tested nominal concentration of the WAF.

Description of key information

The substance has a EL50 >100 mg/L.