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EC number: 600-569-0 | CAS number: 104491-84-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- Deviations:
- yes
- Remarks:
- Inoculum was run alone instead of in duplicate and the test substance was run in duplicate instead of in triplicate.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of 1,5-dihydroxy-4-nitro-8-(phenylamino)anthraquinone and 1,8-dihydroxy-4-nitro-5-(phenylamino)anthraquinone
- EC Number:
- 944-623-4
- Molecular formula:
- C20 H12 N2 O6
- IUPAC Name:
- Reaction mass of 1,5-dihydroxy-4-nitro-8-(phenylamino)anthraquinone and 1,8-dihydroxy-4-nitro-5-(phenylamino)anthraquinone
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Mixture of polyvalent bacteria collected from the aration tank of a domestic sewage treatment plant
- Details on inoculum:
- Mixture of polyvalent bacteria collected on May 20, 1997 from the aeration tank of a domestic sewage treatment plant, PRO RHENO Basel communal.
The pH value of the sludge was 7.7. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 7.3 other: mg/ 250 ml test medium
- Based on:
- ThOD
- Initial conc.:
- 7.5 other: mg/ 250 ml test medium
- Based on:
- ThOD
- Initial conc.:
- 7.6 other: mg/ 250 ml test medium
- Based on:
- ThOD
- Details on study design:
- This method was based on the following conditions:
- test chemicals as sole organic carbon sources
- no pre-adaptation of micro-organisms to the test chemicals
The test was performed in a Voith Sapromat.
Tested chemicals were inoculated in the testing vessels with microorganisms. During the test
period, the Biochemical Oxygen Demand was measured continuousily by means of a BODmeter.
Biodegradability was calculated on the basis of the theoretical oxygen demand (ThOD)
or of the COD, if the ThOD could not be calculated.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- None
- Test performance:
- The test was considered valid because:
- the difference of extremes values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d. window was less than 20%. [ 10-d. window: the 10 days immediately following the attainment of 10% biodégradation.]
- the degradation of the reference substance at day 7: >/= 40%
- the degradation of the reference substance at day 14: >/= 65%
- the abiotic degradation of the steril control at day 28 was not: >10%
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- St. dev.:
- 0
- Sampling time:
- 28 d
BOD5 / COD results
BOD5 / CODopen allclose all
- Parameter:
- BOD5*100/ThOD
- Value:
- 0 other: %
- Parameter:
- BOD5
- Value:
- 0 mg O2/g test mat.
- Results with reference substance:
- Biodegradation of the reference substance after 28 days: 100 %
Any other information on results incl. tables
Reference substance:
The biodegradation of the reference substance corrected by the blank control and measured as BOD versus ThOD was:
92 % after 7 days
97 % after 14 days
100% after 28 days
Abiotic control:
The abiotic degradation measured as BOD was: 4 % after 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- On the basis of the present study the test substance FAT 92504/A was not biodegradable.
- Executive summary:
The ready biodegradability of FAT 92504/A was determined in a 28 days Modified MITI (II) - Test according to the OECD Guideline for Testing of Chemicals No 302C (adopted 1981).
The test substance and the reference substance were tested in a concentration of about 47.10 mg ThOD/30 mg and 167 mg ThOD/100 mg, respectively.
Results:
Mean value of the biodegradation of FAT 92504/A after 28 days: 0 %
Biodegradation of the reference substance after 28 days: 100 %
The biochemical oxygen demand (BOD5) after 5 days was: 0 mg 02/g
Conclusion:
On the basis of the present study the test substance FAT 92504/A was not biodegradable.
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