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Diss Factsheets
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EC number: 440-740-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 MAR 2000 - 08 JUN 2000
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Version / remarks:
- Official Journal of the European Communities Nr. L 383 A, Part C from 29.12.92, Method C.4-E: Closed Bottle Test
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Drainage of the municipal sewage treatment plant of the city of Darmstadt (Südhessische Gas und Wasser AG)
- Preparation of inoculum for exposure: Aeration for 5 days.
- Pretreatment: Filtration through a coarse pleated filter.
- Concentration of sludge: 5 mL/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 50 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20 +/- 1 °C
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 2
SAMPLING
- Sampling frequency: weekly
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes - Reference substance:
- aniline
- Remarks:
- 2.0 mg/L
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 4
- Sampling time:
- 28 d
- Details on results:
- Points of degradation plot (test substance):
3 % degradation after 7 d
2 % degradation after 14 d
4 % degradation after 21 d
4 % degradation after 28 d - Results with reference substance:
- Points of degradation plot (reference substance):
74 % degradation after 7 d
87 % degradation after 14 d
90 % degradation after 21 d
100 % degradation after 28 d - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The study was conducted under GLP according to EU Method C.4E on the registered substance itself with marginal deficiencies in documentation in the test report; the available information allows to draw the conclusion that the test was conducted properly without deviation from the guideline. The method is considered reasonable, the reference substance produced the appropriate response and the validity criteria are met. Hence, the results can be considered as reliable to assess the ready biodegradability of the test substance by sludge retrieved from the drainage of a municipal sewage treatment plant. Within the test period of 28 days, a degradation of 4 % was determined for the test substance. Hence, the test substance has to be classified as "Not Readily Biodegradable".
- Executive summary:
In a study according to EU Method C.4E under GLP, 50 mg/L of the test substance is suspended in a mineral medium, inoculated with a mixed population of aquatic microorganisms ( drainage of a municipal sewage treatment plant) and incubated for 28 days under aerobic conditions in the dark at 20 ± 1°C. During this period, the biodegradation of the test substance is determined on the basis of the reduction of dissolved oxygen.
Within the test period of 28 days, a degradation of 4 % was determined for the test substance.
In parallel preparations with the reference substance aniline a degradation of 87 % was achieved within 14 days.
Accordingly, the test substance has to be classified as "Not Readily Biodegradable".
This biodegradation study is classified as acceptable and satisfies the guideline requirement for a biodegradation study (closed bottle test).
Reference
Description of key information
Biodegradation in water: screening tests: Not readily biodegradable: 4 % (BOD) in 28 days (EU Method C.4 -E, GLP)
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
In a study according to EU Method C.4E under GLP, 50 mg/L of the test substance is suspended in a mineral medium, inoculated with a mixed population of aquatic microorganisms (drainage of a municipal sewage treatment plant) and incubated for 28 days under aerobic conditions in the dark at 20 ± 1°C. During this period, the biodegradation of the test substance is determined on the basis of the reduction of dissolved oxygen.
Within the test period of 28 days, a degradation of 4 % was determined for the test substance.
In parallel preparations with the reference substance aniline a degradation of 87 % was achieved within 14 days.
Accordingly, the test substance has to be classified as "Not Readily Biodegradable".
This biodegradation study is classified as acceptable and satisfies the guideline requirement for a biodegradation study (closed bottle test).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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