ethametsulfuron-methyl (ISO); methyl 2-[(4-ethoxy-6-methylamino-1,3,5-triazin-2-yl)carbamoylsulfamoyl]benzoate

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EEC Guidelines for the Testing of Chemicals, The Artificial Soil Test, EEC 79/831, Rev. 3

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Reference substance 002

Cas Number:

97780-06-8

Test material form:

solid

Details on test material:

- Purity: 96.8%

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

yes

Remarks:

acetone

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test conditions

Test temperature:

20 ± 2°C

pH:

6 ± 0.5

Moisture:

artificial soil had 30% moisture content

Nominal and measured concentrations:

0.1, 1, 10, 100, and 1000 mg/kg (a.s./soil dry weight)

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Results and discussion

Effect concentrations

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Remarks on result:

other: Highest dose tested

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

14 d LC50 (E. fetida): >1000 mg/kg soil dw (mortality) (highest concentration tested)

Executive summary:

The objective of this study was to evaluate the toxicity of the test substance to the earthworm (Eisenia fetida), confined to treated artificial soil for fourteen days. The methods described in this study are consistent with EEC 79/831, Rev. 3. The test substance was tested at concentrations of 0.1, 1, 10, 100, and 1000 mg a.s./kg soil dry weight. The standard, chloroacetamide, was used at concentrations of 2, 4, 8, 16, 32, and 64 mg/kg. Earthworm mortality was determined at 7 and 14 days post treatment.

Soil applications of the test substance had no effect on the earthworm. There was no mortality in any of the test units treated with the test substance. Hence, the LC50 was estimated to be greater than 1000 mg a.s./kg soil dry weight, the highest rate tested. Also, weight loss during the course of the study was no greater than that sustained in the control units. LC50 for the standard, chloroacetamide, was 22.6 mg/kg. There was no mortality in the control units.