4-ethoxybenzoic acid

Endpoint summary

Description of key information

Short term toxicity to fish:

Short term toxicity study of Benzoic acid, 4-ethoxy- on the growth of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus for 24 h Measured concentration of 5 mg/l Chemicals were weighed in calibrated weighing bottles to the nearest milligram on a Volland Speedigram balance. Test performed in the 10 liter of glass battery jar contain 5 liter of water having the headspace of 5 liter. After the exposure of 24 hrs with chemical no any mortality and other effect was observed.

Based on the no effect and mortality of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus, due to the Benzoic acid, 4-ethoxy-, the EC0 was 5 ppm.

Short term toxicity to aquatic invertebrate:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.The stock solution 100 g/l  was prepared by dissolving white powder in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water1 , 2 , 4 , 8 , 16 , 32 mg/lconcentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 21.3 mg/L on the basis of mobility inhibition effects in a 48 hour study.No immobiliation was observed in reconstituted water without the sample , 0 % the daphnids were immobilized in the control + DMSO at the end of test.Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The stock solution 100 mg/l was prepared by dissolving white powder  in DMSO. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.. Various concentration used were10 , 18 , 34 , 60 , 100 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (EC50) for the test substance , in algae was determined to be 127.5 mg/L on the basis of growth rate inhibition effects in a 72 hour stud . Based on the EC50 value, which indicates that the substance is likely to be non-hazardous to aquatic algae and cannot classified as per the CLP classification criteria.

Additional information

Summarized result of toxicity of 4-Ethoxybenzoic acid (619-86-3) on the growth and other activity of aquatic fishes, invertebrates and algae and cyanobacteria by considering the data for target as well as RA chemical which was selected on the basis of structure similarity are as follows:

Short term toxicity to fish:

Short term toxicity study of Benzoic acid, 4-ethoxy- on the growth of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus for 24 h Measured concentration of 5 mg/l Chemicals were weighed in calibrated weighing bottles to the nearest milligram on a Volland Speedigram balance. Test performed in the 10 liter of glass battery jar contain 5 liter of water having the headspace of 5 liter. After the exposure of 24 hrs with chemical no any mortality and other effect was observed.

Based on the no effect and mortality of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus, due to the Benzoic acid, 4-ethoxy-, the EC0 was 5 ppm.

Short term toxicity to aquatic invertebrate:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.The stock solution 100 g/l  was prepared by dissolving white powder in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water1 , 2 , 4 , 8 , 16 , 32 mg/lconcentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 21.3 mg/L on the basis of mobility inhibition effects in a 48 hour study.No immobiliation was observed in reconstituted water without the sample , 0 % the daphnids were immobilized in the control + DMSO at the end of test.Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The stock solution 100 mg/l was prepared by dissolving white powder  in DMSO. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.. Various concentration used were10 , 18 , 34 , 60 , 100 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (EC50) for the test substance , in algae was determined to be 127.5 mg/L on the basis of growth rate inhibition effects in a 72 hour stud . Based on the EC50 value, which indicates that the substance is likely to be non-hazardous to aquatic algae and cannot classified as per the CLP classification criteria.