Registration Dossier
Registration Dossier
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EC number: 212-842-8 | CAS number: 873-55-2
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: refer below principle
- Principles of method if other than guideline:
- By Disk diffusion method, antibacterial and antifungal activities of sulfone derivatives including test compound sodium benzenesulfinate was tested for four bacterial strains (two gram positive, and two gram negative ones) and two yeasts.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material: sodium benzenesulfinate
- Molecular formula (if other than submission substance): C6H6O2S.Na
- Molecular weight (if other than submission substance): 164.16 g/mol
- Smiles notation (if other than submission substance): c1(ccccc1)S(=O)[O-].[Na+]
- InChI: 1S/C6H6O2S.Na/c7-9(8)6-4-2-1-3-5-6;/h1-5H,(H,7,8);/q;+1/p-1
- Substance type: Organic
- Physical state: Solid - Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):DMSO - Test organisms (species):
- Staphylococcus aureus
- Details on inoculum:
- Petri dishes were prepared with a base layer of Mu¨ellere Hinton agar medium (MHA, Becton Dickinson) and a top layer (2 mL) of 0.2% MHA medium inoculated with each bacteria suspension (1e3 105 CFU/mL). After drying, 6 mm diameter disks (biodisks Me´rieux) soaked with 10 mL of different compounds were placed on the agar. Disks containing streptomycin (10 mg), penicillin G (10 U/IE), chloramphenicol (30 mg) and fluconazole (20 mg) were used as positive controls and DMSO (10 mL) as negative control.
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 30 min
- Hardness:
- No data
- Test temperature:
- 37 deg.C
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- 10 μl/disk
- Details on test conditions:
- In Disk diffusion method; paper disk containing title comp. placed on Mueller-Hinton agar plate inoculated with test bacteria ATCC 6538, bacteria ATCC 10536, bacteria ATCC 15442, bacteria ATCC 10541, fungi ATCC 90029,fungi ATCC 90029, strain and incubated at 37 deg C; diameter of inhibition zone measured.
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 30 min
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 other: μl/disk
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: No effects observed for Staphylococcus aureus,Escherichia coli,Candida albicans,Candida tropicalis,Pseudomonas aeruginosa and Enterococcus hirae species
- Results with reference substance (positive control):
- -For Staphylococcus aureus-streptomycin with 27 mm inhibition zone as positive control
-For Escherichia coli-chloramphenicol with 34 mm inhibition zone as positive control
-For Candida albicans-fluconazole with 18 mm inhibition zone as positive control
-For Candida tropicalis-fluconazole with 21 mm inhibition zone as positive control
-For Pseudomonas aeruginosa-penicillin G with 24 mm inhibition zone as positive control
-For Enterococcus hirae-penicillin G with 14 mm inhibition zone as positive control - Validity criteria fulfilled:
- not specified
- Conclusions:
- By Disk diffusion method, antibacterial and antifungal activities of sulfone derivatives including test compound sodium benzenesulfinate was tested for four bacterial strains (two gram positive, and two gram negative ones) and two yeasts.
The minimal inhibitory concentration (MIC) value of the test compound sodium benzenesulfinate was observed at dose concentration 10 μl/disk in DMSO for 30 min. The test compound showed no activity against any of the tested strains (i.e Staphylococcus aureus,Escherichia coli,Candida albicans,Candida tropicalis,Pseudomonas aeruginosa and Enterococcus hirae) whereas the zone of inhibition for the positive controls are in the range 14-34 mm.
Thus, NOEC for the substance can be considered to be 10 μl/disk for 30 minute study. - Executive summary:
By Disk diffusion method, antibacterial and antifungal activities of sulfone derivatives including test compound sodium benzenesulfinate was tested for four bacterial strains (two gram positive, and two gram negative ones) and two yeasts.
In Disk diffusion method; paper disk containing title comp. placed on Mueller-Hinton agar plate inoculated with test bacteria ATCC 6538, bacteria ATCC 10536, bacteria ATCC 15442, bacteria ATCC 10541, fungi ATCC 90029,fungi ATCC 90029, strain and incubated at 37 deg C; diameter of inhibition zone measured as follows:
The zone of inhibition for the positive controls are in the range 14-34 mm as mentioned below:
-For Staphylococcus aureus-streptomycin with 27 mm inhibition zone as positive control
-For Escherichia coli-chloramphenicol with 34 mm inhibition zone as positive control
-For Candida albicans-fluconazole with 18 mm inhibition zone as positive control
-For Candida tropicalis-fluconazole with 21 mm inhibition zone as positive control
-For Pseudomonas aeruginosa-penicillin G with 24 mm inhibition zone as positive control
-For Enterococcus hirae-penicillin G with 14 mm inhibition zone as positive control
According to the disk diffusion method, the minimal inhibitory concentration (MIC) value of the test compound sodium benzenesulfinate was determined at dose concentration 10 μl/disk in DMSO for 30 min. The test compound showed no activity against any of the tested strains (i.e Staphylococcus aureus,Escherichia coli,Candida albicans,Candida tropicalis,Pseudomonas aeruginosa and Enterococcus hirae). Thus, NOEC for the substance can be considered to be 10 μl/disk for the 30 minute study
Reference
Description of key information
Antibacterial and antifungal activities of sulfone derivatives including test compound sodium benzenesulfinate was tested for four bacterial strains (two gram positive, and two gram negative ones) and two yeasts by Disk diffusion method.
The minimal inhibitory concentration (MIC) value of the test compound sodium benzenesulfinate was observed at dose concentration 10 μl/disk in DMSO for 30 min. The test compound showed no activity against any of the tested strains (i.e Staphylococcus aureus,Escherichia coli,Candida albicans,Candida tropicalis,Pseudomonas aeruginosa and Enterococcus hirae) whereas the zone of inhibition for the positive controls are in the range 14-34 mm.
Thus, NOEC for the substance can be considered to be 10 μl/disk for 30 minute study.
Key value for chemical safety assessment
Additional information
By Disk diffusion method, antibacterial and antifungal activities of sulfone derivatives including test compound sodium benzenesulfinate was tested for four bacterial strains (two gram positive, and two gram negative ones) and two yeasts.
In Disk diffusion method; paper disk containing title comp. placed on Mueller-Hinton agar plate inoculated with test bacteria ATCC 6538, bacteria ATCC 10536, bacteria ATCC 15442, bacteria ATCC 10541, fungi ATCC 90029,fungi ATCC 90029, strain and incubated at 37 deg C; diameter of inhibition zone measured as follows:
The zone of inhibition for the positive controls are in the range 14-34 mm as mentioned below:
-For Staphylococcus aureus-streptomycin with 27 mm inhibition zone as positive control
-For Escherichia coli-chloramphenicol with 34 mm inhibition zone as positive control
-For Candida albicans-fluconazole with 18 mm inhibition zone as positive control
-For Candida tropicalis-fluconazole with 21 mm inhibition zone as positive control
-For Pseudomonas aeruginosa-penicillin G with 24 mm inhibition zone as positive control
-For Enterococcus hirae-penicillin G with 14 mm inhibition zone as positive control
According to the disk diffusion method, the minimal inhibitory concentration (MIC) value of the test compound sodium benzenesulfinate was determined at dose concentration 10 μl/disk in DMSO for 30 min. The test compound showed no activity against any of the tested strains (i.e Staphylococcus aureus,Escherichia coli,Candida albicans,Candida tropicalis,Pseudomonas aeruginosa and Enterococcus hirae). Thus, NOEC for the substance can be considered to be 10 μl/disk for the 30 minute study
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