4-[(1-amino-9,10-dihydro-4-hydroxy-9,10-dioxo-2-anthryl)oxy]-N-(3-ethoxypropyl)benzenesulphonamide

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

6 September 2016 and 31 January 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Specific details on test material used for the study:

Identification: FAT 40444/B TE
Appearance/Physical state: Dark red powder
Batch: PCR92X140707 (China)
Purity: 95.4 %
Expiry date: 14 October 2019
Storage conditions: Approximately 4 °C, in the dark

Test item stored at room temperature between 14th and 18th March 2016, before transferring to 4 °C on sponsor’s authority. No impact to test item.

Radiolabelling:

not specified

Test temperature:

Column temperature: 30 ºC

Details on study design: HPLC method:

The test system utilized a high performance liquid chromatograph. A commercially available cyanopropyl reverse phase HPLC column containing lipophilic and polar moieties was used.

Performance of the Test
Preparation of sample solution
Test item (0.1002 g) was diluted to100 mL with acetonitrile to give a concentration of 1.00 x 103 g/L.

Preparation of dead time solution
The dead time was determined by measuring the retention time of formamide (purity* 99.94%, 659 mg/L solution in methanol: purified water (55:45 v/v)).

Preparation of reference standard solutions
Solutions of reference standards (see following table) were prepared in methanol.

Standard Purity (%)* Concentration (mg/L)
Acetanilide 99+ 109
Phenol 99.9 136
Atrazine 99.1 102
Isoproturon >98.0 114
Triadimenol 98.0 107
Linuron 99.7 104
Naphthalene 99 124
Endosulfan-diol 99.9 108
Fenthion 97.9 102
a-Endosulfan 99.6 109
Phenanthrene ≥99.5 105
Diclofop-methyl 99.1 104
DDT 98.7 106

Determination of retention time
The sample, dead time and reference standard solutions were injected in duplicate using the following HPLC parameters:

HPLC System: Agilent Technologies 1200 Series, incorporating autosampler and workstation
Detector type: Ultraviolet (UV)
Column: XSelect HSS Cyano 5 µm (150 x 4.6 mm id)
Column temperature: 30 ºC
Mobile phase: Methanol: pH5.5 aqueous buffer (55:45 v/v)
pH of mobile phase: 5.49
Flow-rate: 1.0 mL/min
Injection volume: 10 µL
UV detector wavelength: dead time and reference standards: 210 nm; sample: 520 nm

Analytical monitoring:

not specified

Details on sampling:

N/A

Sample No.:

#1

Type:

log Koc

Value:

> 5.63 dimensionless

Sample No.:

#2

Type:

log Koc

Value:

> 5.63 dimensionless

Type:

Koc

Value:

> 427 000 dimensionless

Details on results (HPLC method):

Overall log10 Koc: >5.63
Adsorption coefficient: >427000

Details on results (Batch equilibrium method):

The method guidelines state that the measurement of adsorption coefficient should be carried out on substances in their ionized and unionized forms (where appropriate). Testing was therefore required at pH 5.5. As the slope of the calibration curve for the reference standards showed good first order correlation and as the retention times between duplicate injections for each solution was consistent, the HPLC method was considered valid for the determination of adsorption coefficient. Based on the chromatographic data, the test item was considered to be stable during the test procedure. Chromatography showed 3 significant peaks not present within the blank solution. Peaks were at retention times of 6.4, 28.9 and 34.0 minutes. The peak at 6.4 minutes represented 1.7 % of the total area of the test item and provided log10KOC results of 3.15 (KOC 1.40 x 10^3), where as the peak at 34.0 minutes represented 0.73 % of the total area of the test item and provided log10KOC results of >5.63 (KOC >4.27 x 10^5). The test item has a purity of 95 % and therefore the largest peak has been reported.

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient (Koc) of the test item has been determined to be greater than 427000, log10 Koc >5.63. This value indicates that the test item is immobile.

Executive summary:

The adsorption coefficient of FAT 40444/B has been determined using the HPLC screening method, designed to be compatible with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001 Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008. Based on the study results adsorption coefficient of FAT 40444/B is 4.27 x 10⁵, log₁₀ K_oc >5.63. The test item is immobile.

Description of key information

The adsorption coefficient of Disperse Red 092 has been determined to be 4.27 x 10⁵, log₁₀K_oc >5.63.

Key value for chemical safety assessment

Koc at 20 °C:

427 000

Additional information

The adsorption coefficient of FAT 40444/B has been determined using the HPLC screening method, designed to be compatible with Method 121 of the OECD Guidelines for Testing of Chemicals, 22 January 2001 Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008. Based on the study results adsorption coefficient of of FAT 40444/B is 4.27 x 10⁵, log₁₀K_oc >5.63. The test item is immobile. [LogKoc: 5.63]