3-bromoanisole

Endpoint summary

Description of key information

Short-term toxicity to fish:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity to fish was predicted for 1-bromo-3-methoxybenzene (CAS no 2398-37-0). LC50 value was estimated to be 26.39 mg/L on Danio rerio (previous name: Brachydanio rerio) for 96 hrs duration. Based on this value it can be concluded that the 1-bromo-3-methoxybenzene (CAS no 2398-37-0) can be considered as toxic to fish at environmentally relevant concentrations and can be classified in aquatic chronic category 3 as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of 1-bromo-3-methoxybenzene to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 100 mg/l was prepared by dissolving colourless liquid in reconstituted water. Test solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 5, 10, 20, 40 and 80 mg/l test concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Study was consider valid as it fulfills all the necessary criteria and follows the standard guideline. The median effective concentration (EC50) for the test substance 1-bromo-3-methoxybenzene, in Daphnia magna was determined to be 28.4 mg/L on the basis of mobility inhibition effects in a 48 hour study. Thus EC50 value indicates that the substance 1-bromo-3-methoxybenzene is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

 

Toxicity to aquatic algae:

Aim of this study was to evaluate the nature of test chemical, when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with OECD growth medium. Stock solution was kept in ultrasonic bath for 20 min and then inoculated with algae. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (ERC50) for the test substance 1-bromo-3-methoxybenzene, in algae was determined to be 55.6 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the ErC50 value the substance is likely to be hazardous to aquatic algae and can be be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to microorganisms:

The toxicity to micro organism of the substance 1-bromo-3-methoxybenzene (Cas no.2398-37-0) to micro organism Tetrahymena pyriformis is predicted using QSAR toolbox version.3.3, based on the effects observed in a static freshwater system during a 48 hr exposure. The Inhibition growth concentration (IGC50) for the substance 1-bromo-3-methoxybenzene is estimated to be 54.44 mg/L.

Additional information

Short-term toxicity to fish:

Three studies from prediction reports and experimental study from authorative database for short term fish toxicity endpoint for test chemical 1-bromo-3-methoxybenzene (Cas no. 2398-37-0) with relevent read across which is close to target using Log Kow as descriptor were reviewed as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity to fish was predicted for 1-bromo-3-methoxybenzene (CAS no 2398-37-0). LC50 value was estimated to be 26.39 mg/L on Danio rerio (previous name: Brachydanio rerio) for 96 hrs duration. Based on this value it can be concluded that the 1-bromo-3-methoxybenzene (CAS no 2398-37-0) can be considered as toxic to fish at environmentally relevant concentrations.

In another prediction done using the EPI Suite ECOSAR version 1.10 the 96 hours LC50 was estimated to be 20.934 mg/l on Fish for substance 1-bromo-3-methoxybenzene (CAS no.2398-37-0) on the basis of mortality effects. Based on this value, it can be concluded that the test chemical can be considered as toxic to fishat environmentally relevant concentrations.

A supporting experimental study for read across chemical 1,4-dichlorobenzene (Cas no. 106-46-7) from authorative database (ECOTOX database) indicate the short term fish toxicity study on test species Danio rerio (Zebra Danio)  was determined for an exposure period 96 hrs. In the experiment static condition was maintained. On the basis of mortality effect the lethal concentration (LC50) on fish Danio rerio was observed to be 22.2mg/l. Thus considering the lethal concentration the test substance1,4-Dichlorobenzene can be considered as toxic to fishat environmentally relevant concentrations.

All the above studies for target and read across substance indicate that the chemical is likely to be toxic to fish. Thus, based on the available information, the test substance 1-bromo-3-methoxybenzene (Cas no. 2398-37-0) can be considered as toxic to aquatic environment and classisfied in aquatic chronic category 3 as per the CLP criteria.

Short term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of 1-bromo-3-methoxybenzene to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 100 mg/l was prepared by dissolving colourless liquid in reconstituted water. Test solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 5, 10, 20, 40 and 80 mg/l test concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Study was consider valid as it fulfills all the necessary criteria and follows the standard guideline. The median effective concentration (EC50) for the test substance 1-bromo-3-methoxybenzene, in Daphnia magna was determined to be 28.4 mg/L on the basis of mobility inhibition effects in a 48 hour study. Thus EC50 value indicates that the substance 1-bromo-3-methoxybenzene is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

 

Toxicity to aquatic algae:

Aim of this study was to evaluate the nature of test chemical, when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with OECD growth medium. Stock solution was kept in ultrasonic bath for 20 min and then inoculated with algae. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (ERC50) for the test substance 1-bromo-3-methoxybenzene, in algae was determined to be 55.6 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the ErC50 value the substance is likely to be hazardous to aquatic algae and can be be classified as aquatic chronic 3 category as per the CLP classification criteria.

 

Toxicity to microorganisms:

The toxicity to micro organism of the substance 1-bromo-3-methoxybenzene (Cas no.2398-37-0) to micro organism Tetrahymena pyriformis is predicted using QSAR toolbox version.3.3, based on the effects observed in a static freshwater system during a 48 hr exposure. The Inhibition growth concentration (IGC50) for the substance 1-bromo-3-methoxybenzene is estimated to be 54.44 mg/L. Based on this value, it can be concluded that the test chemical 1-bromo-3-methoxybenzene may have concern to micro organism toxicity for acute exposure period.