4-aminomethyl-2-methoxyphenol hydrochloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

information on test item storage was lacking

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2001

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Version / remarks:

1996

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-aminomethyl-2-methoxyphenol hydrochloride

EC Number:

230-468-3

EC Name:

4-aminomethyl-2-methoxyphenol hydrochloride

Cas Number:

7149-10-2

Molecular formula:

C8H11NO2.ClH

IUPAC Name:

4-aminomethyl-2-methoxyphenol hydrochloride

Test material form:

other: solid

Details on test material:

- Physical state: white solid
- Analytical purity: 99.77 %(w/w)
- Lot/batch No.: 77

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

reconstituted water, constituents seee below.
The highest concentration was prepared by dissolving 50mg lof test item in 500mL test water by stirring for 10 min. Lower concentrations were prepared from this solution.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

clone 5 from Umweltbundesamt, Berlin, Germany from 1997
bred in IBACON laboratories
female, 6.5 - 22h old
Acclimatization 6.5h under test conditions

Study design

Test type:

static

Water media type:

other: deionized water, reconstituted

Test conditions

Hardness:

2.5 mmol/L as CaCO3

Test temperature:

21°C

pH:

7.6 - 7.8

Dissolved oxygen:

>8.1mg/L

Salinity:

2.0 mmol/L CaCl2 x 2H2O
0.5 mmol/L MgSO4 X 7H2O
0.75 mmol/L NaHCO3
0.075 mmol/L KCl

Nominal and measured concentrations:

Nominal concentrations (based on measured concentrations in stock solution):  0, 4.6, 10, 21, 46 and 100 mg/L.

Details on test conditions:

16h light (502 lux) : 8h dark

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence limits

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

84 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

no positive control used

Reported statistics and error estimates:

The 24h EC50 value and the 95% confidence value could not be calculated due to the low toxic effect after 24h. The 48h EH50 and the 95% confidence limits were calculated by moving average interpolation.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Vanillylamin-HCl was tested for the mobility respective survival of Daphnia magna: In a static test for 48 hours the EC50 of 84 mg/L has been determined. The EC50 after 24 hours was > 100 m/L.

Executive summary:

The 48 hour EC50 was calculated to be 84 mg test substance/L with 95% confidence limits from 58.4 to 120.8 mg/L, according to OECD TG 202 (1984).