.alpha.-Cyclodextrin, 2-hydroxypropyl ethers

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Cavasol W6 HP

IUPAC Name:

Cavasol W6 HP

Details on test material:

- Name of test material (as cited in study report): CAVASOL W6 HP
- Physical state: solid powder
- Analytical purity: 95.9% (GC)
- Impurities (identity and concentrations): as stated in substance data
- Composition of test material, percentage of components: as stated in substance data
- Lot/batch No.: 63B001
- Expiration date of the lot/batch: 2003-02-28
- Stability under test conditions: stable
- Storage condition of test material: in original container, at room temperature, in the dark

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sample storage conditions before analysis: room temperatur, in the dark

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Cavasol W6 HP was completely soluble in the test medium at the concentrations tested.The lower concentrations were prepared by subsequentdilutions in the test medium.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Age at study initiation (mean and range, SD): less than 3 days old
- Method of breeding: same water quality as in test (pH, main ions, total hardness)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

250 mg/l as CaCO3

Test temperature:

18-22 °C

pH:

7.7 to 7.9

Dissolved oxygen:

at least 8.3 mg/l or higher

Nominal and measured concentrations:

100 mg/l , 10 mg/l, 1 Mg/l and 0.1 mg/l; 92% recovery in the 100 mg/l group

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass beakers of 100 ml filled with 80 ml.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):2
- No. of vessels per control (replicates):2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 at 24 and 48 h

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

none

Reported statistics and error estimates:

Moving average interpolation

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Substance is not toxic to daphnids.

Executive summary:

The acute toxicity of Cavasol W6 HP to Daphnia magna was determined in a 48 hour static test in accordance with OECD Guideline 202. A stock solution with nominal concentration of 100 mg/L was stirred. The undiluted stock solution and their dilutions were tested. The concentration of the substance in the freshly prepared stock solution and after 48 hours has been determined by TOC . The concentration of the hydrolysis product was stable during the test period. An EC50 (48 h) of > 100 mg has been determined.