Tris(triphenylphosphine)rhodium (I) chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP compliant, guideline study, available as unpublished report, acceptable with restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Analytical verification of the test concentration carried out after 0 and 48hr, and analysis of the stock solution was conducted at 0hr.

Vehicle:

no

Details on test solutions:

1 g/L stock solution in synthetic freshwater lacking NaHCO3 was prepared, stirred for about 18 hours and filtered. A single test item concentration (nominal 12.4 mg/L) was used in a limit test.

Test organisms (species):

Daphnia magna

Details on test organisms:

Test organisms (species): Daphnia magna STRAUS, clone 5
Details on test organisms – source: Received from Bayer AG, Leverkusen, Germany then bred in house
Details on test organisms - Holding conditions prior to test: Maintained in M4 medium in 1L beakers. Fed with Scenedesmus subspicatus
Details on test organisms - life stage of test species used: Offspring less than 24 hrs old

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

No data

Hardness:

250mg CaCO3/L

Test temperature:

20 +- 1°C

pH:

7.8 -8.6

Dissolved oxygen:

8.2 - 8.4 mg/l

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal: 12.4 mg/L
Measured concentrations did not deviate by more than 20% of nominal, therefore results are based on nominal concentrations.

Details on test conditions:

Test vessel: round bottomed test tube
Aeration: None
No of organisms per vessel: 5
No of replicates per concentration: 4
Photoperiod: dark
Feeding: none

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 24 and 48h EC50 >12mg/l (>water solubility). 12mg/l was the highest concentration that could be obtained under test conditions

Details on results:

Due to the low water solubility of the test item EC50 values after 24 and 48 h could not be determined

Results with reference substance (positive control):

After 24 hours, 40% immobilisation was seen at 1 mg/L and 100% immobilisation at 2 mg/L. The results demonstrate that the sensitivity of the individuals lies within the normal range.

Validity criteria fulfilled:

yes

Remarks:

Yes. < 10% of organisms had been immobilised or trapped at the surface of the water. DO at the end of the test was > 60% of the air saturation temp used. There was evidence that the concentration of the test item had been maintained during the test

Conclusions:

The 24 and 48 hr EC50 of tris(thiphenylphosphine)rhodium(I)chloride to Daphnia magna was >12mg/l (>water solubility). 12mg/l was the highest concentration that could be obtained under test conditions

Executive summary:

This is a GLP compliant, guideline study and is considered suitable for use as the key study for this endpoint. A limit test was conducted at the limit of solubility. The test concentration was maintained within 80% of nominal throughout the study, based on analysis of DOC.

The 24 and 48 hr EC50 of tris(thiphenylphosphine)rhodium(I)chloride to Daphnia magna was >12mg/l (>water solubility). 12mg/l was the highest concentration that could be obtained under test conditions

Description of key information

The 24 and 48 hr EC50 of tris(thiphenylphosphine)rhodium(I)chloride to Daphnia magna was >12mg/l (>water solubility). 12mg/l was the highest concentration that could be obtained under test conditions.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

12 mg/L

Additional information

Enste-Diefenbach (2002) is a GLP compliant, guideline study and is considered suitable for use as the key study for this endpoint. A limit test was conducted at the limit of solubility. The test concentration was maintained within 80% of nominal throughout the study, based on analysis of DOC.

The 24 and 48 hr EC50 of tris(thiphenylphosphine)rhodium(I)chloride to Daphnia magna was >12mg/l (>water solubility). 12mg/l was the highest concentration that could be obtained under test conditions.