4-(2,6,6-trimethyl-1-cyclohexen-1-yl)butan-2-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

December 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-(2,6,6-trimethyl-1-cyclohexen-1-yl)butan-2-one

EC Number:

241-318-1

EC Name:

4-(2,6,6-trimethyl-1-cyclohexen-1-yl)butan-2-one

Cas Number:

17283-81-7

Molecular formula:

C13H22O

IUPAC Name:

4-(2,6,6-trimethyl-1-cyclohexen-1-yl)butan-2-one

impurity 1

Reference substance name:

rel-4-((1R,6R)-2,2,6-trimethylcyclohexyl)butan-2-one

Cas Number:

60761-23-1

Molecular formula:

C13H24O

IUPAC Name:

rel-4-((1R,6R)-2,2,6-trimethylcyclohexyl)butan-2-one

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Chemical analysis was undergone at the start of the study, after 24hrs into the study and at the end of exposure. The test item concentrations were stable during the test period between 66.7 and 94.9% of the concentrations measured in freshly prepared solutions. Table 6 in the attached report summarises the analytical results.

Test solutions

Vehicle:

no

Details on test solutions:

The study was performed under a semi-static design. The test solution was renewed after 24 hours of exposure.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

14.2°dH

Test temperature:

20.3 - 20. 8°C.
Test solutions were adapted to the test temperature.

pH:

7.9 for the test medium, 7.7-7.8 for the test solutions

Dissolved oxygen:

97%

Reference substance (positive control):

yes

Remarks:

0% nominal concentration

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

5.72 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

2.38 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

48 h

Dose descriptor:

other: EC20

Effect conc.:

3.38 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

ECx values of the parameter "immobility of daphnids" (48h) were based on statistical evaluation of the biological results and mean measured concentrations in mg test item/L.

The results showed that the EC50 was 5.72 mg/L.