1-chloro-2,5-dimethoxybenzene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976-11-11 to 1977-01-19

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable well documented study report which meets basic scientific principles. Study was conducted prior to GLP and OECD guideline implementation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Company guiedeline previous to OECD guideline implementation that is similar to OECD 401.

Deviations:

not applicable

Principles of method if other than guideline:

The liquid test substance was diluted to several concentrations using sesame oil. Each dilution was administered once to female SPF-Wistar rats. The rats were obtained from in-house breeding and had a mean body weight of 109g (ranging from 100-123g). Since pretests didn't show sex-linked differences in sensitivity only female rats were used. 10 rats were used per dose. 16hrs prior to administration food was withdrawn, 2hrs after adminstration food was made available again. The observation period after administration was 14d. During this time all animals had ad libitum access to food (Altromina 1324, Company Altromin GmbH, Lage/Lippe) and tap water. Housing of animals was done in plastic cages with wood shavings and one animal per cage. Lethally intoxicated animals were dissected and macroscopically evaluated. Surviving animals were narcotized and killed at the end of the observational period. Additionally, they were dissected and macroscopically evaluated.

GLP compliance:

no

Remarks:

- Performed prior to GLP implementation

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2,5-dimethoxybenzene

EC Number:

218-267-9

EC Name:

1-chloro-2,5-dimethoxybenzene

Cas Number:

2100-42-7

Molecular formula:

C8H9ClO2

IUPAC Name:

2-chloro-1,4-dimethoxybenzene

Details on test material:

- Name of test material (as cited in study report): CME = Chlorhydrochinondimethyläther (2-Chlor-1,4-dimethoxybenzol)

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

- Rats were obtained from in-house breeding and a mean body weight of 109g (ranging from 100-123g)
- Pretests ruled out any sex-linked differences in sensitivity --> only female rats were used
- 10 rats were used per dose
- 16hrs prior to administration food was withdrawn
- 2hrs after adminstration food was made available again
- Observation period after administration was 14d
- During this time all animals had ad libitum access to food (Altromina 1324, Company Altromin GmbH, Lage/Lippe) and tap water
- Housing of animals was done in plastic cages with wood shavings.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Sesame oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 4%

Doses:

630, 1000, 1600, 2000mg per kg bw

No. of animals per sex per dose:

10 female Wistar rats per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Frequency of weighing: once a week

Statistics:

LD50 were calculated using Probit models (according to Linder and Weber), confidence intervals were calculated according to Cavalli-Sforza.

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

1 659 mg/kg bw

95% CL:

1 503 - 1 830

Mortality:

630 mg/kg bw: 0 out of 10
1000 mg/kg bw: 0 out of 10
1600 mg/kg bw: 4 out of 10
2000 mg/kg bw: 9 out of 10

Clinical signs:

other: Lethally intoxicated animals showed: ataxic gait, squatting posture, clear colored lacrimation, closed eyes, lateral or prone position, narcosis-like condition

Gross pathology:

No abnormal findings

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

LD50: 1659mg/kg bw

Executive summary:

Testing for acute oral toxicity resulted in a LD50 of 1659mg/kg body weight.