7-(diethylamino)-4-methyl-2-benzopyrone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 20 mg of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 24hr stirring.After analytical detection the solubility found to be 10 mg/l. Hence, limit test continued with concentration of 10 mg/l.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.63 cm
- Weight at study initiation (mean and range, SD): 0.1899 g (average)
- Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Duration of housing: 15 days
- Photoperiod: 16 hours light and 8 hours dark
- Diet : Standard brand feed, once daily
- Acclimatization: seven days
- Feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

148.5 mg of CaCO3

Test temperature:

23.6°C

pH:

7.05

Dissolved oxygen:

8.0 mg/l

Nominal and measured concentrations:

10 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 4 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Table 1. Mortality and symptoms:

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3h		6h		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-	-	-	-	-

Table 2: Visible Symptoms

Nominal Concentration (mg/l)

Number of fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Validity criteria fulfilled:

yes

Conclusions:

Based on the effect on mortality of the test fish Danio rerio, the 96 hr LC0 and median lethal concentration (LC50 (96 h)) was determined to be 10 and > 10 mg/l.

Executive summary:

Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.1899 g and average length of 1.63 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 8.0 mg/l, pH 7.54, water temperature 23.5°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. A limit test was performed at a concentration of 10 mg/l (nominal concentrations). Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23.6°C, pH 7.05, hardness of water 148.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. On the basis of effect of test chemical on mortality of the test organism, the 96 hr LC0 and median lethal concentration (LC50 (96 h)) was determined to be 10 and > 10 mg/l. Based on the LC50 value, it can be concluded that the substance is likely to be hazardous to aquatic invertebrate and considered to be classified as aquatic chronic 3 category as per the CLP classification criteria.

Description of key information

Short term toxicity to fish:

Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.1899 g and average length of 1.63 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 8.0 mg/l, pH 7.54, water temperature 23.5°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. A limit test was performed at a concentration of 10 mg/l (nominal concentrations). Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23.6°C, pH 7.05, hardness of water 148.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. On the basis of effect of test chemical on mortality of the test organism, the 96 hr LC0 and median lethal concentration (LC50 (96 h)) was determined to be 10 and > 10 mg/l. Based on the LC50 value, it can be concluded that the substance is likely to be hazardous to aquatic invertebrate and considered to be classified as aquatic chronic 3 category as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

10 mg/L

Additional information

Following different studies includes experimental study for the target chemical and read-across analogues which is extracted by using mechanistic approach and functionally and structurally similar to the target chemical to observe the toxicity of test chemical on fish.

Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.1899 g and average length of 1.63 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 8.0 mg/l, pH 7.54, water temperature 23.5°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. A limit test was performed at a concentration of 10 mg/l (nominal concentrations). Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23.6°C, pH 7.05, hardness of water 148.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control and test vessel. On the basis of effect of test chemical on mortality of the test organism, the 96 hr LC0 and median lethal concentration (LC50 (96 h)) was determined to be 10 and > 10 mg/l. Based on the LC50 value, it can be concluded that the substance is likely to be hazardous to aquatic invertebrate and considered to be classified as aquatic chronic 3 category as per the CLP classification criteria.

Second short term fish toxicity study was conducted for 24 hrs for assessing the lethal effect of read across chemical on test organism. Study was conducted in a static system at 18°C and 7.2 pH. Test was perfomed on Ptychocheilus oregonensis as a test organism.On the basis of effect of test chemical on test organism,the 48 hrs LC50 value was determined to be 10mg/l.

Last short term fish toxicity study was conducted for 24 hrs for assessing the lethal effect of read across chemical on test organism. Study was conducted in a static system at 10°C and 7.2 pH. Test was perfomed on Ptychocheilus oregonensis used as a test organism. On the basis of effect of test chemical on test organism, the 24 hrs LC50 value was determined to be 10mg/l.

On the basis of the above results, it can be concluded that the test chemical was considered to be toxic to aquatic fishes and hence, considered to be classified in 'aquatic chronic category 3' as per the CLP classification criteria.