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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

Currently viewing:

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer-reviewed journal

Data source

Reference
Reference Type:
publication
Title:
A Review of the Toxicity of Twelve Fluorescent Dyes Used for Water Tracing
Author:
P.L. Smart
Year:
1984
Bibliographic source:
The NSS Bulletin, Volume 46 Number 2: 21-33 - October 1984, A publication of the National Speleological Society

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as per mentioned below
Principles of method if other than guideline:
Study is conducted to check the LD50 of Sodium fluorescein after oral administration in rat
GLP compliance:
not specified
Test type:
other: no data
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
EC Number:
208-253-0
EC Name:
Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
Cas Number:
518-47-8
Molecular formula:
C20H12O5.2Na
IUPAC Name:
disodium 3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate
Test material form:
other: Solid
Details on test material:
- Name of test material (IUPAC name): disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate- Common name: Fluorescein sodium- Molecular formula: C20H10Na2O5- Molecular weight:376.274 g/mol- Smiles notation: c1(c2c(oc3c1ccc(c3)[O-])cc(=O)cc2)c1c(cccc1)C(=O)[O-].[Na+].[Na+]- InChl: 1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;;/h1-10,21H,(H,23,24);;/q;2*+1/p-2- Substance type: Organic

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
not specified
Details on oral exposure:
No data available
Doses:
No data available
No. of animals per sex per dose:
No data available
Control animals:
not specified
Details on study design:
Duration of observation period following administration: 14 days (or other?): 14 days
Statistics:
Probit analysis by method of Finney

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
6 720 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 50% mortality
Mortality:
No data
Clinical signs:
other: No data
Gross pathology:
No data available

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated informationCriteria used for interpretation of results: EU
Conclusions:
LD50 was considered to be 6720 mg/kg bw when rats were treated with Sodium fluorescein orally.
Executive summary:

Ina acute oral toxicity study, rat were treated with Sodium fluorescein the concentration of 6720 mg/kg bw. 50 % mortality was observed in treated rats at 6720 mg/kg bw. Therefore, LD50 was considered to be 6720 mg/kg bw when rats were treated with Sodium fluorescein orally.