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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer-reviewed journal

Data source

Reference
Reference Type:
publication
Title:
A Review of the Toxicity of Twelve Fluorescent Dyes Used for Water Tracing
Author:
P.L. Smart
Year:
1984
Bibliographic source:
The NSS Bulletin, Volume 46 Number 2: 21-33 - October 1984, A publication of the National Speleological Society

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as per mentioned below
Principles of method if other than guideline:
Study is conducted to check the LD50 of Sodium fluorescein after oral administration in rat
GLP compliance:
not specified
Test type:
other: no data
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
EC Number:
208-253-0
EC Name:
Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
Cas Number:
518-47-8
Molecular formula:
C20H12O5.2Na
IUPAC Name:
disodium 3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate
Test material form:
other: Solid
Details on test material:
- Name of test material (IUPAC name): disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate- Common name: Fluorescein sodium- Molecular formula: C20H10Na2O5- Molecular weight:376.274 g/mol- Smiles notation: c1(c2c(oc3c1ccc(c3)[O-])cc(=O)cc2)c1c(cccc1)C(=O)[O-].[Na+].[Na+]- InChl: 1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;;/h1-10,21H,(H,23,24);;/q;2*+1/p-2- Substance type: Organic

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
not specified
Details on oral exposure:
No data available
Doses:
No data available
No. of animals per sex per dose:
No data available
Control animals:
not specified
Details on study design:
Duration of observation period following administration: 14 days (or other?): 14 days
Statistics:
Probit analysis by method of Finney

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
6 720 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 50% mortality
Mortality:
No data
Clinical signs:
No data
Body weight:
No data available
Gross pathology:
No data available

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated informationCriteria used for interpretation of results: EU
Conclusions:
LD50 was considered to be 6720 mg/kg bw when rats were treated with Sodium fluorescein orally.
Executive summary:

Ina acute oral toxicity study, rat were treated with Sodium fluorescein the concentration of 6720 mg/kg bw. 50 % mortality was observed in treated rats at 6720 mg/kg bw. Therefore, LD50 was considered to be 6720 mg/kg bw when rats were treated with Sodium fluorescein orally.