p-hydroxyphenylacetic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study is peformed according to OECD testing guideline and GLP, and the report is well described. The presence of substance was measured during the assay.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

p-hydroxyphenylacetic acid

EC Number:

205-851-3

EC Name:

p-hydroxyphenylacetic acid

Cas Number:

156-38-7

Molecular formula:

C8H8O3

IUPAC Name:

p-hydroxyphenylacetic acid

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): p-hydroxyphenylacetic acid
- Physical state: white powder
- Storage condition of test material: in a fume cupboard at 20°C in the dark

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 500 mg/L
- Sampling method: water samples were taken from the middle of the test chamber at study start, and after 48 and 96 hours.
- Sample storage conditions before analysis: no data

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: water for dilution was added to the substance, the mixture homogenised using an Ultra-Turrax and ultrasonic batch and poured into the chamber under stirring with a glass rod.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Strain: Hamilton-Buchanan
- Source: West Aquarium(3422 Bad Lauterberg, FRG)
- Length at study initiation: 2.4 to 3.2 cm (mean: 2.9 cm ± 0.23 cm)
- Method of breeding: -
- Feeding during test: no

ACCLIMATION
- Acclimation period: at least 14 days before the start of the study
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Tetra Min, Tetra Werke, Melle, twice daily ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Test temperature:

22.3 to 22.6°C

pH:

7.2 ot 8.2

Dissolved oxygen:

6.9 to 8.7 mg/L

Nominal and measured concentrations:

Nominal concentrations: 0 and 500 mg/L
Measured concentrations: 499 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10-L test chambers, made out of glass (length 30cm, width 22cm, height 24cm)
- Type (delete if not applicable): no data
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water according to ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Adjustment of pH: yes (adjusted to the pH-value of the dilution water by adding 1.8 mL of NaOH (10 mol/L)
- Photoperiod: 6:00 to 18:00 hrs
- Light intensity: approx. 700 lux

EFFECT PARAMETERS MEASURED:
lethality and visible changes in appearance and behaviour were recorded after 2, 4, 24, 48, 72 and 96 hours.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: -
- Justification for using less concentrations than requested by guideline: limit test at 500 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Appearance and behavioural abnormalities: no changes observed.
- Mortality of control: 0/10
- Other adverse effects control: no changes in appearance and behaviour

No lethality was observed in the 500 mg/L group.

Reported statistics and error estimates:

not applicable

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96-h LC50 of p-hydroxyphenylacetic acid is above 500 mg/L.

Executive summary:

The lethal effect of p-hydroxyphenylacetic acid was evaluated in zebra fish (Brachydanio rerio) over a total of 96 hours in a static test according to OECD testing guideline n° 203. No lethality occured at 500 mg/L (mean measured concentration: 499 mg/L).

The LC50 after 96 hours is therefore above 500 mg/L.