Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 600-813-6 | CAS number: 1072-68-0
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant, similar to guideline study, available as unpublished report, adequate for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,4-Dimethyl-1H-pyrazole
- EC Number:
- 600-813-6
- Cas Number:
- 1072-68-0
- Molecular formula:
- C5H8N2
- IUPAC Name:
- 1,4-Dimethyl-1H-pyrazole
- Details on test material:
- - Name of test material (as cited in study report): 1,4-dimethylpyrazole
- Lot No.: DP9001
- Purity: 99.3%
- Appearance: colorless/light yellow liquid
- Storage: the test substance was stored in a cold, dark place in a test substance storage room
Constituent 1
Method
- Target gene:
- - S. typhimurium: His-locus
- E.coli: Trp-locus
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S9 fraction of phenobarbitone and ß-naphthoflavone induced rats
- Test concentrations with justification for top dose:
- Pre-test: 100, 500, 1000, 2000, 5000 µg/plate
Main test: 313, 625, 1250, 2500, 5000 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: distilled water
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- other: 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide; 2-Methoxy-6-chloro-9-(3-(2-chloroethyl)-amionpropylamino)acridine 2HCl; 2-aminoanthracene
- Remarks:
- With metabolic activation: 2-AA (all strains); Without metabolic activation: 2-(2-Furyl)3-(5-nitro-2-furyl)acrylamide (TA 98, TA 100, WPr2 uvrA), sodium azide (TA 1535), 2-Methoxy-6-chloro-9-(3-(2-chloroethyl)-amionpropylamino)acridine 2HCl (TA 1537)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION : Exposure duration: 48 hours
NUMBER OF REPLICATIONS: 2 test plates per dose or control
DETERMINATION OF CYTOTOXICITY: growth inhibition - Evaluation criteria:
- The main test was valid when the sterility test confirmed the absence of bacterial contamination and when the number of revertant colonies both for the solvent and positive controls was within the range of the background data. Then, the test substance was judged positive when the number of revertant colonies (mean value of two plates) was more than twice that of the solvent control and dose-related. When judgement the reproducibility for the test result was confirmed between a range-finding test and a main test.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
