α,2,4-trichlorotoluene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

α,2,4-trichlorotoluene

EC Number:

202-381-0

EC Name:

α,2,4-trichlorotoluene

Cas Number:

94-99-5

Molecular formula:

C7H5Cl3

IUPAC Name:

2,4-dichloro-1-(chloromethyl)benzene

Test material form:

liquid

Specific details on test material used for the study:

Purity of test material > 99 %

Method

Target gene:

In the Salmonella typhimurium strains (TA 1535, TA 100, TA 1537, TA 1538, TA 98) the amino acid histidine locus is the target gene.
In the Escherichia coli strain (WP2 uvrA) the amino acid tryptophan locus is the target gene.

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Metabolic activation system:

rat liver homogenate fraction( " S-9")

Test concentrations with justification for top dose:

The dose range of 6 different doses from 4 µg/plate to 10 000 µg/plate was used.

Vehicle / solvent:

Acetone

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

9-aminoacridine

2-nitrofluorene

sodium azide

other: N-Methyl-N'-nitro-N-nitrosoguanidine

Details on test system and experimental conditions:

Mutagenicity Test: Top agar is prepared for Salmonella strain. Bacteria are grown overnight in nutrient broth.The suitable amount of bacteria in cell suspension is checked by nephelmetry. For inoculation, stock cultures which are stored at -80 °C are used.

Results and discussion

Test results

Test results 1

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Test results 2

Species / strain:

S. typhimurium, other: TA 100, TA1535, TA1537, TA1538, TA98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Applicant's summary and conclusion

Conclusions:

2,4-Dichlorbenzoyl chloride is not mutagenic in these bacterial test system either with or without exogenous metabolic activiation at the dose level investigated.