1,7-Naphthalenedisulfonic acid, 4-amino-3-[(1E)-2-(2,4-disulfophenyl)diazenyl]-5-hydroxy-6-[(1E)-2-(4-nitro-2-sulfophenyl)diazenyl]-, sodium salt (1:5)

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12th September -17th December 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study was conducted according to OECD and in accordance with GLP. The study material is well characterised

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil

Test material

Test material information

Constituent 1

Reference substance name:

pentasodium 4-amino-3-[(1E)-2-(2,4-disulfonatophenyl)diazen-1-yl]-5-hydroxy-6-[(1E)-2-(4-nitro-2-sulfonatophenyl)diazen-1-yl]naphthalene-1,7-disulfonate

EC Number:

811-858-8

Cas Number:

2149571-68-4

Molecular formula:

C22 H11 N6 O18 S5 .5Na

IUPAC Name:

pentasodium 4-amino-3-[(1E)-2-(2,4-disulfonatophenyl)diazen-1-yl]-5-hydroxy-6-[(1E)-2-(4-nitro-2-sulfonatophenyl)diazen-1-yl]naphthalene-1,7-disulfonate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Radiolabelling:

no

Study design

Test temperature:

A stock solution of the reference substance at a concentration of approximately 1 g/l in methanol was used. The stock solution was diluted to obtain an end solution of 30/70 (v/v) methanol/water. The blank solution for the reference substance was 30/70 (v/v) methanol/water.
Test temperature 35 +/- 1C

HPLC method

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: Acquity UPLC system (Waters, Milford, MA, USA)
- Type: UPLC
- Type, material and dimension of analytical (guard) column: Acquity UPLC HSS Cyano, 100 mm  2.1 mm i.d.,
dp = 1.8 µm (Waters)

- Detection system: Acquity UPLC TUV detector (Waters)


MOBILE PHASES
- Type: 30/70 (v/v) methanol/water

- Experiments with additives carried out on separate columns: yes / no
- pH: Neutral
- Solutes for dissolving test and reference substances: Methanol




REFERENCE SUBSTANCES
- Identity: Acetanilide CAS 103-84-4

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 5 µl

- Quantity of reference substances:
- Intervals of calibration:

REPETITIONS
- Number of determinations: Duplicacte for referecne and test substances, single application for blank solutions.

Results and discussion

Adsorption coefficient

Adsorption coefficient 1

Type:

Koc

Value:

< 18

Temp.:

35 °C

Adsorption coefficient 2

Type:

log Koc

Value:

< 1.26

Temp.:

35 °C

Results: HPLC method

Details on results (HPLC method):

Not given in report

Any other information on results incl. tables

	Koc	Log Koc
Test Substance	<1.8 x 10^1	<1.26

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The HPLC method using soil-adsorption-reference data was applied for the determination of the adsorption coefficient (Koc) of K1600 black dye

Koc = < 1.26 Log Koc =  <1.8 x 10 1