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EC number: 276-857-1 | CAS number: 72812-34-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed OECD study with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Hydrogen [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)
- EC Number:
- 276-857-1
- EC Name:
- Hydrogen [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)
- Cas Number:
- 72812-34-1
- Molecular formula:
- C32H18CrN6O8.C11H25NO.H
- IUPAC Name:
- Chromate(1-), [1-[2-[2-(hydroxy-kO)-4-nitrophenyl]diazenyl-kN1]-2-naphthalenolato(2-)-kO][1-[2-[2-(hydroxy-kO)-5-nitrophenyl]diazenyl-kN1]-2-naphthalenolato(2-)-kO]-, hydrogen, compd. with 3-[(2-ethylhexyl)oxy]-1-propanamine (1:1:1)
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap de Aa', Schijndel, the Netherlands.
The sludge was kept under continuous aeration until further treatment. The concentration of suspended solids was 3.9 g/L in the concentrated sludge. Before use, the sludge was allowed to settle for at least 30 minutes and the liquid decanted for use as inoculum at the amount of 10 ml/L of mineral medium. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 26.7 mg/L
- Based on:
- test mat.
- Initial conc.:
- 15 other: mg TOC/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The test substance was tested in duplicate at 53.4 mg and 53.2 mg per 2 litres, corresponding to 15 mg TOC/l.
The organic carbon content was based on the molecular formula.
Positive control: containing reference substance (ca. 40 mg/l sodium acetate and inoculum.
The test was started by bubbling C02-free air through the solution at a rate of approximately 1-2 bubbles per second (ca. 30-100 ml/min).
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0.7
- Sampling time:
- 2 d
- Remarks on result:
- other: Bottle A
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1.1
- Sampling time:
- 29 d
- Remarks on result:
- other: Bottle A
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1
- Sampling time:
- 2 d
- Remarks on result:
- other: Bottle B
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 10.9
- Sampling time:
- 29 d
- Remarks on result:
- other: Bottle B
BOD5 / COD results
- Results with reference substance:
- The reference substance sodium acetate showed 83.4 % degradation after 29 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- The substance is not readily biodegradable
- Executive summary:
The substance was tested for its ready biodegradability in the Carbon Dioxide (C02) Evolution Test (Modified Surm Test) at 53.4 mg and 53.2 mg per 2 litres, corresponding to 15 mg TOC/1.
The relative degradation values calculated from the measurements performed during the test period revealed no significant (>10%) degradation of the test item in bottle A and very slight degradation (11%) in bottle B. Thus, degradation of the test item of at least 60% was not reached within 10 days of biodegradation exceeding 10%.
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