[8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Principles of method if other than guideline:

To determine Adsorption coefficient (KOC) on soil and on sewage sludge of the test chemical [8-[(4-amino-3-nitrophenyl)azo]-7-
hydroxy-2-naphthyl]trimethylammonium chloride (CAS No. 68391-32-2 )using HPLC.

GLP compliance:

no

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Radiolabelling:

no

Test temperature:

25°C

Details on study design: HPLC method:

HPLC Procedure:
The solutions of the test substance and reference substances were prepared in solvent mentioned. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k(table 3).The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor.

Operating Conditions:
HPLC Model No. : Agilent Technologies,1260 Infinity
Flow Rate : 1.0ml/min
Column : ZORBAX Eclipse Plus C18, 4.6×100mm, 3.5 μm
Mobile phase : ACN : water (55:45)
Temperature : 25°C
Detector : Diode Array Detector @210nm
Injector : Autosampler
Injector volume : 10μl
Data Acquisition software : ChemStationOpen lab Control Panel

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: 400 mg/l
- Sampling interval: A test item solution was prepared by accurately weighing 4 mg of test item and diluted with Acetonitrile up to 10 ml. Thus, the test solution concentration was 400 mg/l. The pH of test substance was 6.5.

Key result

Sample No.:

#1

Type:

log Koc

Value:

1.937 dimensionless

pH:

6.5

Temp.:

25 °C

Remarks on result:

other: The Log Koc value was reported as 1.937± 0.000 at 25°C.

Details on results (HPLC method):

TThe Adsorption coefficient (KOC) on soil and on sewage sludge of the [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride (CAS No. 68391-32-2)was determined to be 1.937± 0.000 at 25°C.

Transformation products:

not specified

Table 1:

Reference substance	Log Koc	Rt1	Rt2	Rt3	Mean Rt ± S.D.	k	Log k
Urea	NA	0.887	0.887	0.888	0.887 ±0.000	0	0
Phenol	1.32	1.455	1.455	NA	1.455±0.000	0.6404	-0.1936
4-methylaniline(p Tolouidine)	1.9	1.755	1.748	NA	1.7515±0.005	0.9746	-0.0112
N-methylaniline	2.28	2.303	2.294	NA	2.2985±0.006	1.5913	0.2018
Aniline	2.07	1.513	1.495	NA	1.504±0.013	0.6956	-0.1576
2 - nitrophenol	2.47	2.213	2.224	NA	2.2185±0.008	1.5011	0.1764
Nitrobenzene	2.35	2.426	2.429	NA	2.4275±0.002	1.7368	0.2397
Xylene	2.58	6.241	6.232	NA	6.2365±0.006	6.031	0.7804
Ethylbenzene	2.649	6.359	6.355	NA	6.357±0.003	6.1669	0.7901
Toluene	2.369	4.378	4.382	NA	4.38±0.003	3.938	0.5953
Naphthalene	2.75	6.127	6.218	NA	6.1725±0.064	5.9589	0.7752
phenanthrene	4.09	11.996	11.779	NA	11.8875±0.153	12.4019	1.0935

Table 2:

Test substance[8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride (CAS No. 68391-32-2)		K	LogK	Log Koc by Generalized Calibration Graph	Mean of Log Koc ± S.D.
tR1	1.794	1.023	0.010	1.936	1.937± 0.000
tR2	1.795	1.024	0.010	1.937

Validity criteria fulfilled:

yes

Conclusions:

The Adsorption Coefficient of test substance [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride (CAS No. 68391-32-2) was determined as per the HPLC method (OECD Guideline-121). The Log Koc value was determined to be 1.937± 0.000 at 25°C.

Executive summary:

The adsorption coefficient Koc in soil and in sewage sludge of test chemical [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride (CAS No. 68391-32-2) was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The solutions of the test substance and reference substances were prepared in appropriate solvents. A test item solution was prepared by accurately weighing 4 mg of test item and diluted with Acetonitrile up to 10 ml. Thus, the test solution concentration was 400 mg/l. The pH of test substance was 6.5. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k(Annex - 2).The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The reference substances were chosen according to functional similarity with the test substance and calibration graph prepared. The reference substances were chosen according to functionally similarity with the test substance and calibration graph prepared. The reference substances were Phenol, 4-methylaniline(p-Tolouidine), N-methylaniline, Aniline, 2 - nitrophenol, Nitrobenzene, Xylene, Ethylbenzene, Toluene, Naphthalene, phenanthrene having Koc value ranging from 1.32 to 4.09. The Log Koc value of test substance was determined to be 1.937± 0.000 at 25°C. This log Koc value indicates that the substance [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride (CAS No. 68391-32-2) has aLow sorption to soil and sedimentand therefore havemoderate migrationpotential to ground water.

Description of key information

The Adsorption Coefficient of test substance  [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride (CAS No. 68391-32-2 was determined as per the HPLC method (OECD Guideline-121). The Log Koc value was determined to be 1.937± 0.000 at 25°C. This log Koc value indicates that the substance has aLow sorption to soil and sedimentand therefore havemoderate migrationpotential to ground water.

Key value for chemical safety assessment

Koc at 20 °C:

86.496

Additional information

The adsorption coefficient Koc in soil and in sewage sludge of test chemical [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride (CAS No. 68391-32-2) was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The solutions of the test substance and reference substances were prepared in appropriate solvents. A test item solution was prepared by accurately weighing 4 mg of test item and diluted with Acetonitrile up to 10 ml. Thus, the test solution concentration was 400 mg/l. The pH of test substance was 6.5. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k(Annex - 2).The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The reference substances were chosen according to functional similarity with the test substance and calibration graph prepared. The reference substances were chosen according to functionally similarity with the test substance and calibration graph prepared. The reference substances were Phenol, 4-methylaniline(p-Tolouidine), N-methylaniline, Aniline, 2 - nitrophenol, Nitrobenzene, Xylene, Ethylbenzene, Toluene, Naphthalene, phenanthrene having Koc value ranging from 1.32 to 4.09. The Log Koc value of test substance was determined to be 1.937± 0.000 at 25°C. This log Koc value indicates that the substance [8-[(4-amino-3-nitrophenyl)azo]-7-hydroxy-2-naphthyl]trimethylammonium chloride (CAS No. 68391-32-2) has aLow sorption to soil and sedimentand therefore havemoderate migrationpotential to ground water.