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Toxicity to aquatic algae and cyanobacteria

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Description of key information

After 72 hours of exposure of the fresh water green alga Chlorella vulgaris  to test item tetrasodium 1-acetamido-2-hydroxy-3-(4-((4-sulphonatophenylazo)-7-sulphonato-1-naphthylazo))naphthalene-4,6-disulphonate(CAS No. 2519-30-4) to various nominal test concentrations, EC50 was found to be 24.342mg/l graphically and through probit analysis.

Key value for chemical safety assessment

EC50 for freshwater algae:
24.342 mg/L

Additional information

The effect of test item tetrasodium 1-acetamido-2-hydroxy-3-(4-((4-sulphonatophenylazo)-7-sulphonato-1-naphthylazo))naphthalene-4,6-disulphonate, CAS No. 2519-30-4 was studied on the growth of fresh water green algaChlorella vulgaris.The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 0.3 mg/L, 0.96 mg/L, 3.072 mg/L, 9.830 mg/L, 31.457 mg/L and 100.663 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 24.342 mg/L.

On the basis of avove reported information, the test substance is likely to result in toxicity category II to aquatic algae as per the CLP criteria.