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EC number: 219-746-5 | CAS number: 2519-30-4
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
After 72 hours of exposure of the fresh water green alga Chlorella vulgaris to test item tetrasodium 1-acetamido-2-hydroxy-3-(4-((4-sulphonatophenylazo)-7-sulphonato-1-naphthylazo))naphthalene-4,6-disulphonate(CAS No. 2519-30-4) to various nominal test concentrations, EC50 was found to be 24.342mg/l graphically and through probit analysis.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 24.342 mg/L
Additional information
The effect of test item tetrasodium 1-acetamido-2-hydroxy-3-(4-((4-sulphonatophenylazo)-7-sulphonato-1-naphthylazo))naphthalene-4,6-disulphonate, CAS No. 2519-30-4 was studied on the growth of fresh water green algaChlorella vulgaris.The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 0.3 mg/L, 0.96 mg/L, 3.072 mg/L, 9.830 mg/L, 31.457 mg/L and 100.663 mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 24.342 mg/L.
On the basis of avove reported information, the test substance is likely to result in toxicity category II to aquatic algae as per the CLP criteria.
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