Disodium 4-[4-[[5-[(2-bromo-1-oxoallyl)amino]-2-sulphonatophenyl]azo]-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl]-2,5-dichlorobenzenesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

other: Rainbow trout

Details on test organisms:

Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen

Further standard conditions:
- Basins have been cleaned with RBS-35 solution (FLUKA 83462) and rinsed with clean water.
- Testing water should be kept at a pH between 6 and 8.5. In case of deviations, pH needs to be adjusted with NaOH or HCl solution.
- Acclimatization period: minimum 12 days at 10-13 °C for trout.
- Feeding periods: daily (TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany)
- Day-night cycles: 12/12h
- Termination: For termination of the experiment MS-222 (Sandoz) should be used at 1 % (Methacain).

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

14 °C

pH:

7.4-8.1

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations: 100, 200 and 1000 mg/L

Details on test conditions:

Water:
- 15 liters for trout, Water used according to ISO guidelines.

Basins:
- Full-glass basins, 17 liters basis (36x23x26 cm) tempered (cold-room); 5 liter basins (15x20x20 cm) tempered

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

570 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was seen at 100 and 200 mg/L concentrations, however at 1000 mg/L, 6 out of 8 fish were found to have died during the 48 hour observation period.

Validity criteria fulfilled:

yes

Conclusions:

The TL50 of FAT 40061/A in this fish acute toxicity test was determined to be 570 mg/L.

Executive summary:

The acute fish toxicity test was performed using a methodology equivalent or similar to OECD Guideline 203. Rainbow trout were used in this test. Groups of fish, each containing 8 fish, were exposed to test concentrations of 100, 200 and 1000 mg/L. No mortality was seen at 100 and 200 mg/L concentrations, however at 1000 mg/L, 6 out of 8 fish were found to have died during the 48 hour observation period. Based on these findings, the 48 h TL50 of FAT 40061/A was determined to be 570 mg/L.

Description of key information

The acute fish toxicity test was performed using a method equivalent or similar to OECD Guideline 203. Rainbow trout were used in this test. Groups of fish, each containing 8 fish, were exposed to test concentrations of 100, 200 and 1000 mg/L.No mortality was seen at 100 and 200 mg/L concentrations, however, at 1000 mg/L, 6 out of 8 fish were found to have died during the 48-hour observation period. Based on these findings, the 48 h TL50 of FAT 40061/A was determined to be 570 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

570 mg/L