2',3,4',5,7-pentahydroxyflavone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2',3,4',5,7-pentahydroxyflavone

EC Number:

207-542-9

EC Name:

2',3,4',5,7-pentahydroxyflavone

Cas Number:

480-16-0

Molecular formula:

C15H10O7

IUPAC Name:

2',3,4',5,7-pentahydroxyflavone

Constituent 2

Reference substance name:

Morin

IUPAC Name:

Morin

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

34.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

53.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

40.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-h EC50 was calculated to be 53.3 mg/L

Executive summary:

Acute toxicity of the test item Morin was assessed on Daphnia magna in a 48-hour Acute Immobilisation Test. Young Daphnia were exposed to aqueous test media containing the test item for 48 hours at a range of concentrations. A concurrent control group was run. Test concentrations investigated in the study were prepared by appropriate diluting of the stock solution. Fully characterized (content, physico-chemical characteristics) ISO Medium was used as dilution water. Based on the results of the preliminary experiment, the calculated geometric mean test item concentrations (based on the analytical measurements) of 3.9, 9.3, 19.2, 40.5 and 81.2 mg/L were tested in the main study. Healthy, young female Daphnids with known origin, history and acclimatisation conditions were treated in the test. Twenty Daphnia (divided into four replicates) were tested in each concentration and untreated control. Each test vessel contained approximately 40 mL test medium. Environmental conditions were measured with the suitable instruments. All of the measured values remained in the acceptable ranges. The immobilisation of the test animals was recorded 24 and 48 hours after treatment.

After 24 hours of exposure no immobility and any abnormal behaviour was observed in the control group and in concentrations up to 40.5 mg/L. Immobility was observed only in the highest concentration of 81.2 mg/L (35 % immobility). At the end of the test (48h exposure) no immobility and any abnormal behaviour was observed in the control group and in concentrations up to 19.2 mg/L. In the two highest concentrations (40.5 and 81.2 mg/L) 20 % and 90 % immobility was noticed respectively. Accordingly, the 48-h NOEC was determined to be 19.2 mg/L The 48-h EC50 was calculated to be 53.3 mg/L.