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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro DNA damage and/or repair study
Remarks:
Type of genotoxicity: other: mitotic recombination
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Not specified; published in 1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Actual concentrations tested and number of replicates are not reported, results of positive controls are not reported, and the test was not repeated.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1988

Materials and methods

Test guideline
Qualifier:
no guideline followed
Deviations:
not applicable
Principles of method if other than guideline:
No guideline was stated in the report, but the study complies with the TNsG on in vitro gene mutation and is largely compliant with OECD 481
GLP compliance:
no
Type of assay:
mitotic recombination assay with Saccharomyces cerevisiae

Test material

Constituent 1
Chemical structure
Reference substance name:
Hydrogen chloride
EC Number:
231-595-7
EC Name:
Hydrogen chloride
Cas Number:
7647-01-0
Molecular formula:
Cl H
IUPAC Name:
hydrogen chloride
Details on test material:
Hydrochloric acid
Specification: Fisher Certified
Purity: 95% or greater
No further details specified.

Method

Target gene:
Not applicable.
Species / strain
Species / strain / cell type:
other: Saccharomyces cerevisiae strain D4
Details on mammalian cell type (if applicable):
Nutritional deficiencies due to heteroallelism at the adenine-2 and tryptophan-5 locus.
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
S9 derived from liver of rats previously treated with Aroclor 1254.
Test concentrations with justification for top dose:
0.001-5 microlitre/plate
Vehicle / solvent:
no data
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
yes
Remarks:
Appropriate solvent (absolute ethanol) controls were used, but not reported.
True negative controls:
no
Positive controls:
yes
Remarks:
Appropriate positive controls were used, but not reported.
Positive control substance:
not specified
Details on test system and experimental conditions:
Way of application: The procedure followed (Brusick and Andrews, 1974) parallels the Ames/Salmonella assay with several modifications.

Method of application: in agar (plate incorporation)
Exposure Duration: 3-5 days
Incubation temperature: 30C - S9 mix, 37C + S9 mix
Number of cells evaluated: Approximately 10^7 added to plate.
Number of replicants: not specified.

Evaluation criteria:
The result would be considered positive for induction of mitotic recombination if the test item produced a positive dose response over three concentrations with the highest increase equal to 2-3 times the solvent control value
Statistics:
no data

Results and discussion

Test results
Species / strain:
Saccharomyces cerevisiae
Metabolic activation:
with and without
Genotoxicity:
negative
Remarks:
with/without metabolic activation
Cytotoxicity / choice of top concentrations:
other: Not reported
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
Actual concentrations and number of replicates tested are not reported, results of positive controls are not reported, and the test was not repeated.
Remarks on result:
other: other: Saccharomyces cerevisiae strain D4
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Negative results were obtained both with and without metabolic activation. 

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative The result would be considered positive for induction of mitotic recombination if the test item produced a positive dose response over three concentrations with the highest increase equal to 2-3 times the solvent control value

Hydrochloric acid is not mutagenic under these test conditions.