Registration Dossier
Registration Dossier
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EC number: 231-987-8 | CAS number: 7783-28-0
Toxicity to other aquatic organisms
Administrative data
- Endpoint:
- toxicity to other aquatic vertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-standard test.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Toxicity of simazine, diammonium phosphate and rock phosphate to fish, snail and worm at various water temperatures.
- Author:
- Sarkar, S.K.
- Year:
- 1 997
- Bibliographic source:
- Geobios 24:142-145.
- Reference Type:
- publication
- Title:
- Standard Methods for the Examination of Water and Wastewater.
- Author:
- American Public Health Association (APHA)
- Year:
- 1 985
- Bibliographic source:
- Washington, D.C.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: APHA (1985)
- Deviations:
- not specified
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Diammonium hydrogenorthophosphate
- EC Number:
- 231-987-8
- EC Name:
- Diammonium hydrogenorthophosphate
- Cas Number:
- 7783-28-0
- Molecular formula:
- H3N.1/2H3O4P
- IUPAC Name:
- diammonium hydrogen phosphate
- Details on test material:
- Diammonium phosphate ((NH4)2HPO4), commercial grade
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- other: Viviparus bengalensis (snail) and Branchiura sowerbyi (worm)
Study design
- Test type:
- other: Static, Open-system
- Water media type:
- not specified
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No data
Test conditions
- Hardness:
- No data
- Test temperature:
- 21 °C / 26 °C / 31 °C / 36 °C
- pH:
- 7.5
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- No data
- Details on test conditions:
- Bioassay tests were conducted on snails and worms after methods described in APHA (1985). Tests were made in 100 mL glass beakers at pH 7.5. Each concentration was tested in four replicates accompanied by controls, with either six snails or ten worms per beaker (i.e., 24 snails and 40 worms per concentration).
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2 427 mg/L
- Remarks on result:
- other: V. bengalensis; 21°C
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2 450 mg/L
- Remarks on result:
- other: V. bengalensis; 26°C
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1 850 mg/L
- Remarks on result:
- other: V. bengalensis; 31°C
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1 005 mg/L
- Remarks on result:
- other: V. bengalensis; 36°C
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2 455 mg/L
- Remarks on result:
- other: B. sowerbyi; 21°C
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2 005 mg/L
- Remarks on result:
- other: B. sowerbyi; 26°C
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1 740 mg/L
- Remarks on result:
- other: B. sowerbyi; 31°C
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1 200 mg/L
- Remarks on result:
- other: B. sowerbyi; 36°C
- Details on results:
- The toxicity of DAP to both species increased with increasing water temperature. In addition, in tests in which snails and worms were exposed to both DAP and the pesticide simazine, the presence of DAP fertilizer reduced the toxicity of simazine.
Applicant's summary and conclusion
- Conclusions:
- LC50 (V. bengalensis; 21°C) = 2,472 mg/L
LC50 (V. bengalensis; 26°C) = 2,450 mg/L
LC50 (V. bengalensis; 31°C) = 1,850 mg/L
LC50 (V. bengalensis; 36°C) = 1,005 mg/L
LC50 (B. sowerbyi; 21°C) = 2,455 mg/L
LC50 (B. sowerbyi; 26C) = 2,005 mg/L
LC50 (B. sowerbyi; 31C) = 1,740 mg/L
LC50 (B. sowerbyi; 36C) = 1,200 mg/L
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