Alcohols, C12-13, branched and linear, ethoxylated, sulfates, sodium salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

08 - 12 Feb 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

no guideline followed

Principles of method if other than guideline:

Test is conducted according to internal SOP (106 01) which is not described in the study report

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 10.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 10.0 - 18.0 mg/L

Sublethal observations / clinical signs:

Nominal concentration [mg/L]	Test period (h)
	24 h	48 h	72 h	96 h
	Mortality [%]	Mortality [%]	Mortality [%]	Mortality [%]
Control	0	0	0	0
3.2	0	0	0	0
5.6	0	0	0	0
10.0^*	0	11	11	44
17.9	100	100	--	--
32.0	100	--	--	--

^*One fish escaped from the exposure vessel.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

12 - 16 Sep 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: EG Guideline 92/69 C.1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 3 cm +/- 0.5 cm
- Weight at study initiation (mean and range, SD): approximately 0.25 g
- Feeding during test
- Food type: no feeding


ACCLIMATION
- Acclimation period: 14 days
- Type and amount of food: TetraMin at a concentration of 1% of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): <= 5% seven days prior test until test start

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

11 ° dH

Test temperature:

19 °C - 20 °C

pH:

8.0 - 8.2

Dissolved oxygen:

80% - 99%

Nominal and measured concentrations:

nominal: 4.3, 6.0, 8.5, 11.8 and 17.1
mean measured (over 96 h): 4.6, 6.8, 13.9, 12.1 and 17.2

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: approximately 45 L aquaria
- Aeration: continuously
- Renewal rate of test solution (frequency/flow rate): approximately 10 L/h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 10



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water - Gelsenwasser AG


OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h darkness

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

7.1 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Justification for type of information:

Please refer to the Category Approach Justification provided in Section 13.

Reason / purpose for cross-reference:

read-across source

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

7.1 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Remarks:

Source, WoE, 68891-38-3, 1995b

Description of key information

In the key-study a LC50-value for fish of 7.1 mg a.s./L could be determined after 96 h after exposure. 
Nevertheless that endpoint is not used for PNEC-calculation as PNEC-values were calculated on the basis of a QSAR-model for chronic toxicity. 

Additional information

The assessment is based on the data currently available. New studies, based on the category review and the final decisions issued for some of the category substances, which are also relevant for this assessment, are currently being conducted. The hazard assessment with respect to aquatic toxicity will be updated once all ongoing studies have been finalised.

One GLP-study is available for AES (C12-13, 1-2.5EO) Na (CAS 161074-79-9). In that study rainbow trout were tested with a mixture containing 3 ethoxy groups. The test was conducted according to a standard operating procedure which is not described in the study. In the semi-static study an LC50 -value of approximately 10.4 mg/L was determined. As the substance tested in the study contains low amounts of active substance (26.5%) and the method is not described in the study, additional read-across to data available for the alcohols C12 -14 ethoxysulfates (2EO) is prepared. The read-across study is conducted under GLP according to the guidelines OECD 203 and EG 92/69 C.1. In that test zebrafish (Danio rerio) were exposed to different concentrations of the mixture in a flow-through system (Sasol, 1995b). The resulting LC50 value is reported to be 7.1 mg a.s./L. Both studies were used for a weight of evidence approach.

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