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Acute toxicity studies are available for fish daphnia and algae. For fish the 96h LC50 is >100 mg/L. For daphnia the 48h LC50 for piperazine is observed to be 105.4 mg/L (95% CI: 86.6 - 127.2 mg/L). For Algae 72h ErC50 and ErC10 for piperazine is observed to be 153.1 and 109.3 mg/L respectively.

From the available data Daphnia appear to be the most sensitive aquatic species. The value for long-term toxicity to Daphnia which resulted in a NOEC of 12.5 mg/L is used for calculation of PNEC.

The long-term toxicity to fish is derived using the Acute-to-Chronic (ACR) approach. Based on the available 96-h LC50 > 100 mg/L (NITE, 2003) a NOEC for the long-term toxicity to fish of > 1 mg/L was derived.

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