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EC number: 235-921-9 | CAS number: 13048-33-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- [volume of 0.05 ml applied (OECD: volume of 0.1 ml); 2 animals were used (OECD: 3); observation period was 8 days (OECD: 21); examination using fluorescein (OECD: no use of fluorescein)]
- Principles of method if other than guideline:
- BASF-TEST:
Before OECD guideline 405 was established, eye irritation was tested using an internal method. White Vienna rabbits were used. Usually approximately 50 µl of the test substance were applied to the conjunctival sac of one eye of each of 2 animals. The non- or saline- treated eye served as control. In studies with solids, talcum powder was used as control. The animals were observed several times on the treatment day and up to 8 days afterwards, and findings were recorded on working days. The eyes were not washed out after 24 h as specified in OECD Guideline 405. - GLP compliance:
- no
- Remarks:
- ; GLP was not compulsory at the time the study was conducted
Test material
- Reference substance name:
- Hexamethylene diacrylate
- EC Number:
- 235-921-9
- EC Name:
- Hexamethylene diacrylate
- Cas Number:
- 13048-33-4
- Molecular formula:
- C12H18O4
- IUPAC Name:
- hexane-1,6-diyl bisacrylate
- Details on test material:
- 1,6-Hexanedioldiacrylate (technical grade)
- Name of test material (as cited in study report): HDA2
- Physical state: liquid
- Analytical purity: technical grade
- Stability under test conditions: stabilized, spontaneous degradation or polymerization is not expected
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 3.09 kg, 2.81 kg
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: the second eye of the same rabbit was treated with 50 µl NaCl saline
- Amount / concentration applied:
- undiluted
Amount applied: 0.05 ml - Duration of treatment / exposure:
- 24 h; the substance was not washed out
- Observation period (in vivo):
- 8 days
- Number of animals or in vitro replicates:
- 2 animals, 1 male and 1 female
- Details on study design:
- 50 µl of the undiluted test substance was applied to the right conjuntival sac of one male and one female Vienna White rabbit. The left eye treated with physiological saline served as a control. The substance was not washed out.
Ocular reactions were recorded 10 min, 1, 3 and 24 hours, as well as 2, 5, 7 and 8 days after instillation using a BASF-scoring system, which is convertible into the current OECD Draize scheme. For the evaluation, the BASF scores given in the raw data were converted to Draize scores. The reading time points used for assessment were 24, 48 and 72 hours after application. At day 8, the eyes were additionally examined by using fluorescein.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 24/48 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48 h
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48 h
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- 24/48 h
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 24/48 h
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48 h
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48 h
- Score:
- 0
- Max. score:
- 4
- Other effects:
- Details on irritation response:
In both tested animals secretion was observed in the substance-treated eye up to 3 hours after instillation of the test substance.
Both animals showed slight redness of conjunctivae during the first 5 days (see table 1). However, this effect was fully reversible within 7 days. Cornea and iris were inconspicious.
The left eye of both animals which was treated with saline and served as control showed no effects.
Any other information on results incl. tables
Table 1: Eye irritation parameters; the scores of the BASF study were converted according to the method of Draize
Reading time point |
Animal 1, treated eye (right) |
||||
Cornea score |
Iris score |
Conjunctiva Redness |
Conjunctiva Chemosis |
Additional findings |
|
10 min |
0 |
0 |
1 |
0 |
secretion |
1 h |
0 |
0 |
1 |
0 |
secretion |
3 h |
0 |
0 |
1 |
0 |
secretion |
24 h |
0 |
0 |
1 |
0 |
|
48 h |
0 |
0 |
1 |
0 |
|
5 d |
0 |
0 |
1 |
0 |
|
7 d |
0 |
0 |
0 |
0 |
|
8 d |
0 |
0 |
0 |
0 |
|
Reading time point |
Animal 2, treated eye (right) |
||||
Cornea |
Iris |
Conjunctiva Redness |
Conjunctiva Chemosis |
Additional findings |
|
10 min |
0 |
0 |
1 |
0 |
secretion |
1 h |
0 |
0 |
1 |
0 |
secretion |
3 h |
0 |
0 |
1 |
0 |
secretion |
24 h |
0 |
0 |
1 |
0 |
|
48 h |
0 |
0 |
1 |
0 |
|
5 d |
0 |
0 |
1 |
0 |
|
7 d |
0 |
0 |
0 |
0 |
|
8 d |
0 |
0 |
0 |
0 |
|
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of the test, the test material does not fulfil the requirements to be classified according to EU (as conjunctivae score < 2.5) or GHS (as conjunctivae score < 2) criteria.
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