Allyl 2,3-epoxypropyl ether

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restriction (missing strain for crosslinking/oxidizing agents)

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1990

Reference 2

Reference Type:

publication

Title:

Comparative mutagenicity of aliphatic epoxides in Salmonella.

Author:

Canter DA, Zeiger E, Haworth S, Lawlor T, Mortelmans K, Speck W

Year:

1986

Bibliographic source:

Mutat. Res. 172: 105-138

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

missing strain for crosslinking/oxidizing agents

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Allyl 2,3-epoxypropyl ether

EC Number:

203-442-4

EC Name:

Allyl 2,3-epoxypropyl ether

Cas Number:

106-92-3

Molecular formula:

C6H10O2

IUPAC Name:

2-[(prop-2-en-1-yloxy)methyl]oxirane

Details on test material:

- Name of test material (as cited in study report): Allyl Glycidyl Ether
- Physical state: clear colorless liquid
- Analytical purity: 99 %

Method

Target gene:

his+

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

S9 from Aroclor 1254-induced male Sprague-Dawley rat and Syrian hamster liver

Test concentrations with justification for top dose:

0, 100, 333, 1000, 3333, 10000 µg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Controls 1

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene for all strains

Remarks:

with S9 mix

Controls 2

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: 4-nitro-o-phenylenediamine for TA98, sodium azide for TA100 and TA1535, and 9-aminoacridine for TA1537

Remarks:

without S9 mix

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation

DURATION
- Preincubation period: 20 minutes

METABOLIC ACTIVATION:
The test was conducted with and without metabolic activation. In this study metabolic activation enzymes and cofactors from Aroclor 1254-induced male Sprague-Dawley rat and Syrian hamster liver were used.

REPEAT STUDY: One repeat was performed for TA 100 and TA 1535.

Evaluation criteria:

- A positive response was defined as a reproducible, dose-related increase in histidine-independent (revertant) colonies in any one strain/activation combination.
- An equivocal response was defined as an increase in revertants which was not dose related, not reproducible, or of insufficient magnitude to support a determination of mutagenicity.
- A response was considered negative when no increase in revertant colonies was observed after chemical treatment.

Results and discussion

Test results

Test results 1

Species / strain:

other: Salmonella typhimurium TA100, TA1535

Metabolic activation:

with and without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

10000 µg/plate

Vehicle controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

other: Salmonella typhimurium TA1537, TA98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

10000 µg/plate in TA1537, from 3333 µg/plate in TA98 without S9

Vehicle controls validity:

valid

Positive controls validity:

valid

Additional information on results:

TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No precipitation was observed at any of the doses tested

Others: Mutagenic response was observed in every positive control tested. The solvent control did not induce any mutagenic response.

Remarks on result:

other: other: Salmonella typhimurium TA100, TA1535

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Mean Revertants (±SE) from three plates

TA1535

Dose	No Activation (Positive)		No Activation (Positive)		10% HLI (Positive)		10% HLI (Positive)		10% RLI (Positive)		10% RLI (Positive)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	23	3.8	26	2.7	10	1.8	10	1.5	18	1.2	13	2.3
100	63	10.2	54	5.4	9	1.2	17	2.2	13	0.6	17	3.5
333	125	10.3	166	1	27	3.8	42	5.2	19	7.2	29	2.1
1000	250	45.1	347	9	119	9.1	195	5.7	49	9.8	128	7.2
3333	759	20.2	783	22	444	27.8	516	12	442	43.6	495	12.2
10000	525s	84.6	1019s	26.4	454s	15.3	577s	11.9	521	33.2	406s	91.8
Positive Control	1373	108.5	1604	61.8	115	13.6	139	1.7	119	0.7	63	0.6

 

TA100

Dose	No Activation (Positive)		No Activation (Positive)		10% HLI (Positive)		10% HLI (Positive)		10% RLI (Positive)		10% RLI (Positive)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	105	1.7	159	9	105	9.8	130	5.8	116	13.9	135	5.8
100	177	2.9	211	7.9	84	10.7	137	6.8	123	1.5	143	7.8
333	326	15.3	371	9	147	8.8	172	0.9	161	8.4	155	10.7
1000	904	198.3	814	30.5	329	14.3	399	5.8	382	13.6	417	10.5
3333	2178	119.4	2047	79.8	1382	21.7	1571	14.9	1478	50.1	1388	150.7
10000	3312s	135.3	3431s	15.9	3569	85.7	3705s	91.3	3726	133.3	3654s	63.6
Positive Control	2407	63.5	2397	25.7	2160	50.6	1491	37	1125	41.3	395	17.1

 

TA98

Dose	No Activation (Negative)		10% HLI (Negative)		10% RLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	16	1.8	28	2.3	29	3.1
100	18	2.1	27	3.6	28	1.2
333	19	0.9	25	2.7	27	2
1000	18	1.2	26	1.5	31	5.5
3333	17s	1.2	25	5.8	29	2.6
10000	t		34	5.5	30	2.6
Positive Control	1579	37.9	970	40.2	874	39.3

 

TA1537

Dose	No Activation (Negative)		10% HLI (Negative)		10% RLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	7	0.7	6	0	7	1
100	7	2.3	8	1.5	10	3.2
333	8	2.6	8	1	7	2.1
1000	8	1.3	11	2.2	4	1.5
3333	9	1.7	7	0.6	5s	1
10000	5s	1.2	11	0.9	10s	2.4
Positive Control	346	115	76	20.7	114	13.2

 

RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
s = Slight Toxicity; t = Toxic