Dialuminium nickel tetraoxide

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 Feb - 11 Mar 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Secrétariat général du groupe interministeriel des produits chimiques, DGE-Simap, 12, rue Villiot, 75572 Paris cedex 12, France

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dialuminium nickel tetraoxide

EC Number:

234-454-8

EC Name:

Dialuminium nickel tetraoxide

Cas Number:

12004-35-2

Molecular formula:

(Ni[1-x]*Alx)(Nix*Al[2-x])O4 with n in a range of 0.6 -0.93

IUPAC Name:

dialuminium(3+) nickel(2+) tetraoxidandiide

Details on test material:

- Name of test material (as cited in study report): Nickel Aluminate
- Physical state: solid, blue powder
- Analytical purity: > 99%
- Lot/batch No.: 19044-36; Identification code number: PH-09/0042
- Expiration date of the lot/batch: Sep 2010
- Storage condition of test material: room temperature
- Production date: Oct 2008

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Elevage JANVIER (53940 Le Genest St Isle, France)
- Age at study initiation: 8 weeks
- Weight at study initiation: 187 - 209 g
- Fasting period before study: 1 day
- Housing: groups of 3, clear polycarbonate cages with sawdust bedding and stainless steel mesh lid
- Diet: M20 rat/mouse maintenance diet, ad libitum
- Water: tap water ad libitum
- Acclimation period: at least five days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 - 25
- Humidity (%): 30 - 70
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Amount of vehicle (if gavage): 10 mL/kg bw
- Purity: distilled


MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg


CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: limit test according to OECD 423

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

6

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: examination daily, weighing on day 0 (prior to administration), then on days 2, 7 and 14.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Effect levels 1

Sex:

female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

female

Dose descriptor:

other: LD50 cut-off

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality occured.

Clinical signs:

other: No test substance-related clinical signs were observed. However, a decrease in spontaneous activity was observed in 1/6 animals at 30 min after test substance administration. The animal recovered a normal behaviour 1 hour after administration.

Gross pathology:

Macroscopical examination of the animals did not reveal treatment-related changes.