Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993-05-19 to 1993-05-21
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
The Dissolved Organic Carbon (DOC) concentration of the stock solution used to prepare the test media was determined.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: The test substance was added to water at 1 g/L and stirred for 18 hours. After that the solution was filtered and the DOC determined. 
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM

- Common name: Daphnia magna

- Strain: Clone 5

- Source: Stock culture

- Age at study initiation (mean and range, SD): <24 h

- Method of breeding: Parthenogenetic reproduction

- Feeding during test: None

ACCLIMATION

- Acclimation conditions (same as test or not): yes

- Type and amount of food: sufficient to be consumed each day leaving no residue

- Feeding frequency: daily
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
The sum of the Ca2+ and Mg2+ ions was 2.5 mmol
Test temperature:
20 +/-1ºC
pH:
7.0 - 7.3
Dissolved oxygen:
6.6 - 7.9
Salinity:
Not applicability
Nominal and measured concentrations:
Nominal concentrations (calculated from measured DOC concentration in the stock solution (522 mg/L; equivalent to 1107 mg/L of test substance): 0 (Control), 33.2, 66.4, 110.7, 188.2, 332.1, 575.6, and 996.3 mg/L
Details on test conditions:
TEST SYSTEM

- Test vessel:

- Type: open

- Material, size, headspace, fill volume: glass cylinder containing 10 ml of test medium

- Aeration: none

- No. of organisms per vessel: 5

- No. of vessels per concentration (replicates): 4

- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Synthetic water was used for this test, which consisted of the following:
CaCl2 x 2 H2O at 294 mg/l
MgSO4 x 7 H2O at 113 mg/l
NaHCO3 at 63 mg/l
KCl at 5.5 mg/l  

- Intervals of water quality measurement: end of test

OTHER TEST CONDITIONS

- Photoperiod: Dark

EFFECT PARAMETERS MEASURED: Immobilization after 24 and 48 h exposure

TEST CONCENTRATIONS

- Spacing factor for test concentrations: 1.7 - 2.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
111 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
314 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 246 - 400
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
996 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
612 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 463-809
Details on results:
- Immobilisation of control: 0
Results with reference substance (positive control):
- 24-hour EC50: >0.90, <1.9 mg/L
Reported statistics and error estimates:
EC50 values and their 95% confidence intervals were determined by Probit Analysis

Table 1. Test results

 Nominal test substance concentration (mg/L)  Mean percentage immobilisation after 24 hours    Mean percentage immobilisation after 48 hours
 0 (Control)  0  0
 33.2  0  5
 66.4  0  5
 110.7  0
 188.2  5  20
 332.1  10  60
 575.6  40  80
 996.3  85  100
Validity criteria fulfilled:
yes
Conclusions:
A 48-hour EC50 value of 314 mg/L and NOEC of =111 mg/L have been determined for the effects of the test substance on mobility of Daphnia magna, based on nominal concentrations. Due to the rapid hydrolysis half life of the substance. is likely that the test organisms were primarily exposed to the hydrolysis products of the substance. These results are equivalent to EC50 119 mg/L and NOEC =42 mg/L when expressed in terms of the silanol hydrolysis product.

Description of key information

Short-term toxicity to aquatic invertebrates: 48 hour EC50 314 mg/L (nominal) (EU method C.2). The EC50 is equivalent to 119 mg/L when expressed in terms of the silanol hydrolysis product.

The reported 48 hour EC50 for 2-methoxyethanol for freshwater invertebrates is 9400 mg/L for the reproduction of Brachionus sp. (rotifer).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
119 mg/L

Additional information

A 48-h EC50 value of 314 mg/L (nominal) has been determined for the effects of tris(2-methoxyethoxy)(vinyl)silane on mobility of Daphnia magna. In view of the test media preparation method/exposure regime it is likely that the test organisms were exposed predominantly to the hydrolysis products of the tested substance.

The results may be expressed in terms of concentration of the hydrolysis product, vinylsilanetriol, by applying a molecular weight correction: (MW of silanol = 106.15 / MW of parent = 280.40) * 314 mg/L = 119 mg/L.

This study was assigned a reliability score of 1 as it is a guideline study (EU Method C.2) and was conducted in accordance with GLP.

A 48-hour EC50 of 9400 mg/l (nominal) has been determined for the effects of 2-methoxyethanol on the reproduction ofBrachionus sp.,in a reliable study (Devillers et al., 2002).

Refer to IUCLID Section 6 endpoint summary for further discussion of the approach to chemical safety assessment for this registration substance.