Reaction mixture of hydrogenated tallow alkyl amines with sebacic acid and lithium hydroxide

Endpoint summary

Additional information

Biodegradation in water

Two studies have been performed. A Manometric Respirometry Test according to Directive 92/69/EEC method C.4-D and OECD TG 301-F over a period of 28 days and a Modified CO2 evolution test according to ISO 14593 and ISO 10634 to determine the ready biodegradability of sebacic acid distearylamide:

Modified CO2 evolution test

A study was conducted according to ISO 14593 and ISO 10634 to determine the ready biodegradability of sebacic acid distearylamide as a model component as required by EU Authorities. The inorganic carbon results for sebacic acid distearylamide, show that it achieved a maximum biodegradation of 39 %. The total CO2 evolution in the inoculum blank at the end of the test did not exceed 3.0 mg C/L, or 15 % of the carbon added as test substance, as required by the ISO guideline. After day three the inorganic carbon concentrations in the toxicity control bottles were higher than the sum of the concentrations in the sodium benzoate and sebacic acid distearylamide bottles, indicating the sebacic acid distearylamide did not have a toxic effect. The results from the positive control bottles, after correction for the solvent control, are consistently higher than those in the sodium benzoate bottles, indicating the solvent and emulsifier were not inhibitory to the bacteria. Sebacic acid distearylamide achieved a maximum biodegradation of 39 %, and therefore cannot be considered "readily biodegradable". According to ECHA Guidance on Information requirements and CSR, R7b, Biodegradation above 20% of theoretical (measured as BOD, DOC removal or COD) may be regarded as evidence of inherent, primary biodegradability. Thus, the test item is considered as inherently biodegradable. Since the study was conducted with the one component of the submitted substance respectively the most difficult part to be degraded, the result can be adopted for the substance as such. Consequently, the substance is not expected to be persistent in the environment.

Manometric Respirometry Test

The test substance was investigated for its biodegradability in a Manometric Respirometry Test over a period of 28 days according to OECD guideline 301F and EU method C.4D. The biodegradation was followed by the oxygen uptake of the micro organisms during exposure. As reference compound aniline was tested simultaneously under same conditions as the test substance, functioned as a procedure control. After correction of the main biochemical oxygen demand of the inoculum controls at the end of the end of the 28 -day exposure period was found to be biodegradable by 28.8 % and 26.2 %, based on ThODNH4 under the test conditions. The percentage biodegradation did not exceed 60 % within the 10 -day window. The test substance can therefore not be considered to be ready biodegradable.