1,2-dichlorobenzene

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Well documented publication, study was conducted to a method which is comparable to the screening test of the current guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

Screening test: Filter paper contact test

Deviations:

not specified

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

Worms were grown in the laboratory at room temperature (20 to 22 °C) on a consistent organic food source (horse manure).

Study type:

laboratory study

Substrate type:

filter paper

Limit test:

no

Test temperature:

20 °C

Details on test conditions:

Worms were kept in a darkened incubator

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.021 mg/cm²

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 0.019 - 0.023

Conclusions:

In the so-called contact test, in which the lethal toxicity to the earthworm was determined 48 hours after topical exposure to the substance applied to filter paper, the LC50 for o-dichlorobenzene on the earthworm (Eisenia fetida SAVIGNY) was calculated to be 21 µg/cm2 filter paper (95% confidence range: 19 -23 µg/cm2).

Executive summary:

Neuhauser et al (1985).

In the so-called contact test, in which the lethal toxicity to the earthworm was determined 48 hours after topical exposure to the substance applied to filter paper, the LC50 for o-dichlorobenzene on the earthworm (Eisenia fetida SAVIGNY) was calculated to be 21 µg/cm2 filter paper (95% confidence range: 19 -23 µg/cm2).

Reference 2

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Well documented publication, study was conducted to a method which is comparable to the screening test of the current guideline

Qualifier:

according to guideline

Guideline:

other: EEC Guidelines for the Testing of Chemicals: The Contact and Artificial Soil Tests. EEC 79/831, Rev 3, European Economic Community, Brussels, Belgium (1982)

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

Screening test: Filter paper contact test

Deviations:

not specified

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

Worms were obtained from stock cultures in the laboratory of the authors. Cultures are maintained at room temperature (21 to 24 °C), with cocoons being harvested every two weeks. Aerobically digested domestic sludge was used as the meintenance organic food substrate.

Study type:

laboratory study

Substrate type:

filter paper

Limit test:

no

Test temperature:

20 °C

Details on test conditions:

Worms were kept in a darkened incubator

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.021 mg/cm²

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 0.019 - 0.023

Conclusions:

In the so-called contact test, in which the lethal toxicity to the earthworm was determined 48 hours after topical exposure to the substance applied to filter paper, the LC50 for o-dichlorobenzene on the earthworm (Eisenia fetida SAVIGNY) was calculated to be 21 µg/cm2 filter paper (95% confidence range: 19 -23 µg/cm2).

Executive summary:

Neuhauser et al (1986).

In the so-called contact test, in which the lethal toxicity to the earthworm was determined 48 hours after topical exposure to the substance applied to filter paper, the LC50 for o-dichlorobenzene on the earthworm (Eisenia fetida SAVIGNY) was calculated to be 21 µg/cm2 filter paper (95% confidence range: 19 -23 µg/cm2).

Description of key information

The 48 h LC50 for 1,2-dichlorobenzene on the earthworm (Eisenia fetida SAVIGNY) was calculated to be 21 µg/cm2 (95% confidence range: 19 -23 µg/cm2) by Neuhauser (1985, 1986) according to OECD 207.

Additional information

In the so-called contact test, in which the lethal toxicity to the earthworm was determined 48 hours after topical exposure to the substance applied to filter paper, the LC50 for 1,2-dichlorobenzene on the earthworm (Eisenia fetida SAVIGNY) was calculated to be 21 µg/cm2 filter paper (95% confidence range: 19 -23 µg/cm2) (Neuhauser (1985, 1986)).

This study was carried out according to the screening test (filter paper contact test) outlined in OECD 207 and therefore no toxicity endpoint was reported.