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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 10.11.1981 to 23.12.1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test method was equivalent to OECD guideline 404. No GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1981
Report date:
1981

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Deviations:
no
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Calcium di(acetate)
EC Number:
200-540-9
EC Name:
Calcium di(acetate)
Cas Number:
62-54-4
Molecular formula:
C2H4O2.1/2Ca
IUPAC Name:
calcium diacetate
Details on test material:
- Name of test material (as cited in study report): Calciumacetat

Test animals

Species:
rabbit
Strain:
Vienna White
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Gaukler; 6050 Offenbach/ Main.
- Weight at study initiation: Male median weight: 2.87 kg; Female median weight: 3.07 kg.
- Housing: Animals were housed individually in stainless steel cages, with an area of 40 cm x 51 cm.
- Diet (e.g. ad libitum): Ovator Solikanin 4 mm (Muskator Works 4000 Duesseldorf -1); Daily: 130 g per animal.
- Water (e.g. ad libitum): Daily: ca. 250 mL per animal.

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-26 ºC
- Humidity (%): 30-70 %
- Photoperiod (hrs dark / hrs light): 12 hours light/ 12 hours dark

IN-LIFE DATES: From 10.11.1981 to 19.11.1981

Test system

Type of coverage:
occlusive
Preparation of test site:
shaved
Vehicle:
water
Controls:
not required
Amount / concentration applied:
Application form: 50% aqueous solution
Duration of treatment / exposure:
Exposure time: 4 hours
Observation period:
Observation period: 9 days.
Determinations at 30-60 minutes, 24 h, 48 h, and 9 days after test substance application.
At the end of the study, all animals were sacrificed and pathologically examined.
Number of animals:
3 animals were used (2 males and 1 female).
Details on study design:
TEST SITE
- Area of exposure: 2.5 cm x 2.5 cm

REMOVAL OF TEST SUBSTANCE
- Washing (if done): At the end of the time exposure, the test substance was removed with Lutrol, or with Lutrol and water (1:1).

SCORING SYSTEM: : By Draize: Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics. In Dermal Toxicity pp. 46-59. Austin, TEX: Association of Food and Drug Official of the United States, Texas, State Department of Health.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
edema score
Basis:
animal #1
Time point:
other: 24 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
animal #2
Time point:
other: 24 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
animal #3
Time point:
other: 24 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
animal #1
Time point:
other: 48 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
animal #2
Time point:
other: 48 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
animal #3
Time point:
other: 48 h
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
animal #1
Time point:
other: 9 d
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
animal #2
Time point:
other: 9 d
Score:
0
Max. score:
0
Irritation parameter:
edema score
Basis:
animal #3
Time point:
other: 9 d
Score:
0
Max. score:
0
Irritation parameter:
other: redness
Basis:
animal #1
Time point:
other: 24 h
Score:
0
Max. score:
0
Irritation parameter:
other: redness
Basis:
animal #2
Time point:
other: 24 h
Score:
1
Max. score:
1
Reversibility:
fully reversible within: 48 h
Irritation parameter:
other: redness
Basis:
animal #3
Time point:
other: 24 h
Score:
0
Max. score:
0
Irritation parameter:
other: redness
Basis:
animal #1
Time point:
other: 48 h
Score:
0
Max. score:
0
Irritation parameter:
other: redness
Basis:
animal #2
Time point:
other: 48 h
Score:
0
Max. score:
0
Irritation parameter:
other: redness
Basis:
animal #3
Time point:
other: 48 h
Score:
0
Max. score:
0
Irritation parameter:
other: redness
Basis:
animal #1
Time point:
other: 9 d
Score:
0
Max. score:
0
Irritation parameter:
other: redness
Basis:
animal #2
Time point:
other: 9 d
Score:
0
Max. score:
0
Irritation parameter:
other: redness
Basis:
animal #3
Time point:
other: 9 d
Score:
0
Max. score:
0

Any other information on results incl. tables

Calcium Acetate did not produce any irritation effect on the rabbit skin.

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Calcium Acetate did not produce any irritation effect on the rabbit skin.
Executive summary:

The irritation and corrosive effects of Calcium Acetate acid was studied on Vienna White rabbits (two males and one female).

Animals were exposed for 4 hours to a 50 % aqueous solution and observed for 9 days.

Calcium Acetate did not produce any irritation effect on the rabbit skin.