Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 295-253-9 | CAS number: 91994-95-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://www.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This study is rated "reliable with restrictions" because this study was not conducted according to GLP, but was conducted similar to OECD TG 403, with deviations.
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in Section 13.
Cross-reference
- Reason / purpose for cross-reference:
- read-across: supporting information
Reference
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This study is rated "reliable with restrictions" because this study was not conducted according to GLP, but was conducted similar to OECD TG 403, with deviations.
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- yes
- Remarks:
- Animals were exposed for 6 hours versus 4 hours as outlined in the guidance
- GLP compliance:
- no
- Test type:
- standard acute method
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratory
- Age at study initiation: Not provided
- Weight at study initiation: 175 to 200 grams
- Fasting period before study: Data not provided
- Housing: Animals were housed in groups of five.
- Diet (e.g. ad libitum): Purina Cubed diet, ad libitum
- Water (e.g. ad libitum): Tap water, ad libitum
- Acclimation period: 7 days - Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- other: Specific exposure information is not provided. An assessment of the information provided in the report indicates that the whole animal was placed in the exposure chamber
- Vehicle:
- other: Air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure chamber volume: 112 Litres
- Method of holding animals in test chamber: Data not provided
- Source and rate of air: 15 L/min
TEST ATMOSPHERE
- Brief description of analytical method used: The nominal concentration of the test chemical was determined based on the weight of the material used during 360 minutes with an airflow of 40L/min.
- Samples taken from breathing zone: No
- Analytical verification of test atmosphere concentrations:
- no
- Duration of exposure:
- ca. 6 h
- Concentrations:
- Nominal concentration of the test material was determined to be 28 mg/L.
- No. of animals per sex per dose:
- 10 males
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: All animals were observed during exposure and daily thereafter until study termination; animals were weighed prior to exposure and on day 7 and day 14.
- Necropsy of survivors performed: Yes
- Other examinations performed: Clinical signs, body weight, and histopathology - Statistics:
- Statistical analysis, if performed, is not provided in the study report.
- Sex:
- male
- Dose descriptor:
- other: LT50
- Effect level:
- ca. 265 other: minutes
- Exp. duration:
- 6 h
- Remarks on result:
- other: LT50 data is for rats.
- Mortality:
- All ten rats died during the six hour exposure duration.
- Clinical signs:
- other: Rats exhibited hypoactivity and dyspnoea at 45 minutes of exposure. The sequence of events included hyperactivity, hypoactivity, ataxia, salivation, dyspnoea, tremors, tonic or clonic-tonic convulsions interspersed with periods of mortality. Rats also ex
- Body weight:
- All control animals and surviving animals gained body weight in a normal manner during the post exposure period.
- Gross pathology:
- Animals that died during the exposure consistently showed moderate to severe haemorrhage in large areas of the lungs. Rats also exhibited extremely dark livers. Control animals sacrificed at study termination also exhibited a slight incidence of dark foci in the lungs.
- Interpretation of results:
- other:
- Remarks:
- Not classified
- Conclusions:
- All ten rats died during the six hour exposure period. Based on this mortality data, it may be inferred that the test material is lethal to male Sprague-Dawley rats a concentration of 28 mg/L.
- Executive summary:
In an acute inhalation toxicity assay, ten male Sprague-Dawley rats were exposed to nonene vapours for six hours. All animals were weighed prior to exposure and again on day 7 and 14. Animals were observed for gross signs of toxicity during exposure and daily until study termination. Surviving animals were sacrificed at study termination and all animals, including animals that died prior to study termination, were necropsied and all organs were examined for macroscopic changes. Lungs, kidneys, liver and trachea were stored for histopathological examination if needed.
Several toxicological effects including death were observed during the 6 hour exposure period. All ten rats died during the 6 hours exposure period. Rats exhibited hypoactivity and dyspnoea at 45 minutes of exposure. The sequence of events included hyperactivity, hypoactivity, ataxia, salivation, dyspnoea, tremors, tonic or clonic-tonic convulsions interspersed with periods of mortality. Rats also exhibited clonic convulsions and fasiculations. All control animals and surviving animals gained body weight in a normal manner during the course of the study. Animals that died during the exposure consistently showed moderate to severe haemorrhage in large areas of the lungs. Rats exhibited extremely dark livers. Control animals sacrificed at study termination also exhibited a slight incidence of dark foci in the lungs. Based on the mortality data, it may be inferred that the test material is lethal to rats at a concentration of 28 mg/L. The study authors reported a LT50 of 265 minutes for rats.
This study received a Klimisch score of 2 and is classified as “reliable with restrictions” because this study was not conducted according to GLP, but was conducted similar to OECD TG 403, with deviations.
Several toxicological effects including death were observed during the 6 hour exposure period. All ten rats died during the 6 hours exposure period. Rats exhibited hypoactivity and dyspnoea at 45 minutes of exposure. The sequence of events included hyperactivity, hypoactivity, ataxia, salivation, dyspnoea, tremors, tonic or clonic-tonic convulsions interspersed with periods of mortality. Rats also exhibited clonic convulsions and fasiculations. All control animals and surviving animals gained body weight in a normal manner during the post exposure period. Animals that died during the exposure consistently showed moderate to severe haemorrhage in large areas of the lungs. Rats also exhibited extremely dark livers. Control animals sacrificed at study termination also exhibited a slight incidence of dark foci in the lungs.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- yes
- Remarks:
- Animals were exposed for 6 hours versus 4 hours as outlined in the guidance
- GLP compliance:
- no
- Test type:
- standard acute method
Test material
- Reference substance name:
- Alkenes, C8-10-branched, C9-rich
- EC Number:
- 307-301-9
- EC Name:
- Alkenes, C8-10-branched, C9-rich
- Cas Number:
- 97593-01-6
- IUPAC Name:
- Alkenes, C8-10-branched, C9-rich
- Details on test material:
- - Name of test material (as cited in study report): Nonene MRD-76-42
- Substance type: Alkenes, C8-10-branched, C9-rich
- Physical state: Clear liquid with aromatic odour
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratory
- Age at study initiation: Not provided
- Weight at study initiation: 175 to 200 grams
- Fasting period before study: Data not provided
- Housing: Animals were housed in groups of five.
- Diet (e.g. ad libitum): Purina Cubed diet, ad libitum
- Water (e.g. ad libitum): Tap water, ad libitum
- Acclimation period: 7 days
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- other: Specific exposure information is not provided. An assessment of the information provided in the report indicates that the whole animal was placed in the exposure chamber
- Vehicle:
- other: Air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure chamber volume: 112 Litres
- Method of holding animals in test chamber: Data not provided
- Source and rate of air: 15 L/min
TEST ATMOSPHERE
- Brief description of analytical method used: The nominal concentration of the test chemical was determined based on the weight of the material used during 360 minutes with an airflow of 40L/min.
- Samples taken from breathing zone: No
- Analytical verification of test atmosphere concentrations:
- no
- Duration of exposure:
- ca. 6 h
- Concentrations:
- Nominal concentration of the test material was determined to be 28 mg/L.
- No. of animals per sex per dose:
- 10 males
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: All animals were observed during exposure and daily thereafter until study termination; animals were weighed prior to exposure and on day 7 and day 14.
- Necropsy of survivors performed: Yes
- Other examinations performed: Clinical signs, body weight, and histopathology - Statistics:
- Statistical analysis, if performed, is not provided in the study report.
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- other: LT50
- Effect level:
- ca. 265 other: minutes
- Exp. duration:
- 6 h
- Remarks on result:
- other: LT50 data is for rats.
- Mortality:
- All ten rats died during the six hour exposure duration.
- Clinical signs:
- other: Rats exhibited hypoactivity and dyspnoea at 45 minutes of exposure. The sequence of events included hyperactivity, hypoactivity, ataxia, salivation, dyspnoea, tremors, tonic or clonic-tonic convulsions interspersed with periods of mortality. Rats also ex
- Body weight:
- All control animals and surviving animals gained body weight in a normal manner during the post exposure period.
- Gross pathology:
- Animals that died during the exposure consistently showed moderate to severe haemorrhage in large areas of the lungs. Rats also exhibited extremely dark livers. Control animals sacrificed at study termination also exhibited a slight incidence of dark foci in the lungs.
Any other information on results incl. tables
Several toxicological effects including death were observed during the 6 hour exposure period. All ten rats died during the 6 hours exposure period. Rats exhibited hypoactivity and dyspnoea at 45 minutes of exposure. The sequence of events included hyperactivity, hypoactivity, ataxia, salivation, dyspnoea, tremors, tonic or clonic-tonic convulsions interspersed with periods of mortality. Rats also exhibited clonic convulsions and fasiculations. All control animals and surviving animals gained body weight in a normal manner during the post exposure period. Animals that died during the exposure consistently showed moderate to severe haemorrhage in large areas of the lungs. Rats also exhibited extremely dark livers. Control animals sacrificed at study termination also exhibited a slight incidence of dark foci in the lungs.
Applicant's summary and conclusion
- Interpretation of results:
- other:
- Remarks:
- Not classified
- Conclusions:
- All ten rats died during the six hour exposure period. Based on this mortality data, it may be inferred that the test material is lethal to male Sprague-Dawley rats a concentration of 28 mg/L.
- Executive summary:
In an acute inhalation toxicity assay, ten male Sprague-Dawley rats were exposed to nonene vapours for six hours. All animals were weighed prior to exposure and again on day 7 and 14. Animals were observed for gross signs of toxicity during exposure and daily until study termination. Surviving animals were sacrificed at study termination and all animals, including animals that died prior to study termination, were necropsied and all organs were examined for macroscopic changes. Lungs, kidneys, liver and trachea were stored for histopathological examination if needed.
Several toxicological effects including death were observed during the 6 hour exposure period. All ten rats died during the 6 hours exposure period. Rats exhibited hypoactivity and dyspnoea at 45 minutes of exposure. The sequence of events included hyperactivity, hypoactivity, ataxia, salivation, dyspnoea, tremors, tonic or clonic-tonic convulsions interspersed with periods of mortality. Rats also exhibited clonic convulsions and fasiculations. All control animals and surviving animals gained body weight in a normal manner during the course of the study. Animals that died during the exposure consistently showed moderate to severe haemorrhage in large areas of the lungs. Rats exhibited extremely dark livers. Control animals sacrificed at study termination also exhibited a slight incidence of dark foci in the lungs. Based on the mortality data, it may be inferred that the test material is lethal to rats at a concentration of 28 mg/L. The study authors reported a LT50 of 265 minutes for rats.
This study received a Klimisch score of 2 and is classified as “reliable with restrictions” because this study was not conducted according to GLP, but was conducted similar to OECD TG 403, with deviations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)