2-(2H-benzotriazol-2-yl)-4-(1,1,3,3-tetramethylbutyl)phenol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989-05-25 to 1989-09-13

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP study according to OECD guideline 203. Deviations to the updated OECD Guideline (adopted 1992): no analysis of test concentration, age of fish was not specified, range of size of fish was outside the recommended range

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

adopted 1984

Deviations:

yes

Remarks:

, no stock solution was prepared; fish size: 27 mm - 32 mm (average 30 mm)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: No stock solution was prepared, the test substance and 4 mg/Lof the vehicle were added directly to the water tank to produce the desired test concentrations and homogeneously distributed. The fish were then transfered to the aquaria.
- Controls: Blank: water; Vehicle: 4 mg alkylphenol-polyglykol-ether per liter water
- Chemical name of vehicle: alkylphenol-polyglykol-ether
- Concentration of vehicle in test medium: no stock solution was prepared, 4 mg/L in vehicle control and test concentrations
- Evidence of undissolved material: a slight deposit was observed at concentrations of 18 - 100 mg/L (nominal) from the start of the test

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra-fish
- Source: West Aquarium / D-3422 Bad Lauterberg
- Length at study initiation: mean 30 mm, range 27 -32 mm
- Weight at study initiation: mean 0.24 g; range 0.19 - 0.32 g
- Loading: 0.16 g/L
- Feeding during test: none


ACCLIMATION
- Acclimation period: 12 days
- Adaption: 24 hours / no food 24 hours prior to exposure

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

179 mg CaCO3/L

Test temperature:

23 ± 1°C

pH:

7.8 - 8.6

Dissolved oxygen:

93 -99 %

Nominal and measured concentrations:

nominal: 10, 18, 32, 58 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria
- Material, size, headspace, fill volume: glass aquaria of 20 liter filled with 15 litres (36/22/25 cm)
- Aeration: gentle aeration during the test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water (carbon filter)
- Intervals of water quality measuremen: pH, temperature and Oxygen saturation daily

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 hours daily, Flourescent light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, swimming behaviour, loss of equilibrium, respiratory function, Exophtalmus, Pigmentation

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Test concentrations: 10, 18, 32, 58 and 100 mg/L (nominal)

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: Slight effects on swimming behaviour after 48, 72 and 96 hours at nominal concentration of 100 mg/L
- Mortality of control: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: a slight deposit was observed at concentration 18 - 100 mg/L from the start of the test
- Effect concentrations exceeding solubility of substance in test medium: Yes

Description of key information

With high probability acutely not harmful to fish. No toxic effects occur within the range of water solubility. 

Additional information

In a 96-h acute toxicity study, zebra-fish (Brachydanio rerio) were exposed to the test item at nominal concentrations of 0 (control and solvent control), 10, 18, 32, 58 and 100 mg/L under static conditions. 4 mg/L alkylphenol-polyglykol-esther was used as solvent control and added to the test concentrations. The 96-h LC50 was >100 mg/L, only slight effects on swimming behaviour were observed in the highest test concentration after 48, 72 and 96 hours. It can be concluded that the test substance is not acutely toxic to fish in the range of solubility.