3-methylbut-3-en-1-ol

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1968-09-24

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment; pre-GLP study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Principles of method if other than guideline:

Before OECD Guideline 404 was established in 1984, skin irritation was tested using an internal method (BASF Test); the procedure of which is similar to OECD TG 404.

GLP compliance:

no

Remarks:

pre-GLP study

Test material

Test material information

Constituent 1

Reference substance name:

3-methylbut-3-en-1-ol

EC Number:

212-110-8

EC Name:

3-methylbut-3-en-1-ol

Cas Number:

763-32-6

Molecular formula:

C5H10O

IUPAC Name:

3-methylbut-3-en-1-ol

Details on test material:

- Name of test material (as cited in study report): 3-methyl-3-buten-1-ol
- Analytical purity: 99%
No further data

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

No further data.

Test system

Type of coverage:

occlusive

Preparation of test site:

not specified

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin sites of the same animals

Duration of treatment / exposure:

1, 5, and 15 minutes (short-term test);
20 hours (long-term test)

Number of animals:

2 (short-term test);
2 (long-term test):
totally 4 animals

Details on study design:

TEST SITE
- Area of exposure: back (short-term test and long term test); inner auricle (long-term test); see freetext

REMOVAL OF TEST SUBSTANCE
- Washing (if done): water, possibly containing a mild detergent
- Time after start of exposure: at the end of the respective exposure period

SCORING SYSTEM:
BASF scoring system, convertible to Draize system

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24 h

Score:

1

Max. score:

1

Reversibility:

fully reversible within: 8 days

Results 2

Irritation parameter:

erythema score

Basis:

mean

Time point:

48 h

Remarks on result:

not measured/tested

Results 3

Irritation parameter:

erythema score

Basis:

mean

Time point:

72 h

Remarks on result:

not measured/tested

Results 4

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 8d

Score:

0

Max. score:

1

Reversibility:

fully reversible

Results 5

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Remarks on result:

not measured/tested

Irritant / corrosive response data:

24-hour reading:
In one animal, questionable redness was observed at the dorsal skin that had been treated with the test substance for one minute; no signs of irritation were noted at the respective skin site of the other animal. Questionable and slight redness was observed at the dorsal areas treated for 5 minutes and longer in one animal each. Slight redness was noted at the auricular skin of both animals.

8-day reading:
No signs of irritation were observed at any skin site.

Any other information on results incl. tables

Table:  Skin findings and scores (converted to Draize system)

 

Application site	Exposure time	Findings at 24 h		Findings at 8 d
		Animal no. 1	Animal no. 2	Animal no. 1	Animal no. 2
Back	1 min 5 min 15 min	Erythema: 0 Erythema: 1 Erythema: 1	No findings Erythema: 0 Erythema: 0	No findings at any treated site	No findings at any treated site
Back	20 h	Erythema: 1	Erythema: 0
Ear	20 n	Erythema: 1	Erythema: 1

 

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: OECD GHS

Conclusions:

The test substance was not a primary skin irritant under the conditions of this test.