Sodium 3-(allyloxy)-2-hydroxypropanesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

, difference in range-finding test slightly above 2 °C (+0.2 °C). This slight deviation did not affect the result of the range-finding study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
- Sampling method: 3 mL samples were taken at t=0 and t=96
- Sample storage conditions before analysis: storage in a freezer (stability under conditions was determined in previous project)

Vehicle:

no

Details on test solutions:

Preparation of test solution started with the highest test concentration of nominal 400 mg/L. This concentration corresponded to 100 mg a.i./L. No special treatment other than a short period of magnetic stirring (15 minutes) was applied to ensure complete dissolution in the test medium. The pH of this solution was above 9.0 (9.4 and 9.5, respectively in range-finding and final test) and as a consequence adjusted to 7.8 using 1M HCl (Merck, Darmstadt, Germany). The lower test concentrations were prepared by subsequent dilutions in test medium. The final test solutions were all clear and colourless.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: carp
- Strain: Linnaeus 1758
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen, University and Research Centre, NL
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): range-finding: 2.4 ± 0.2 cm, final test: 2.6 ± 0.2 cm
- Weight at study initiation (mean and range, SD): range-finding: 0.43 ± 0.09 g, final test: 0.53 ± 0.10 g
- Method of breeding:
- Feeding during test
- Food type: -
- Amount: -
- Frequency: no feeding from 24 hours prior to the test and during the total test period

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): adjusted ISO medium
- Type and amount of food: pelleted fish food (Nutra 3.0 T, TROUW nutrition, Putten, NL)
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no information availble

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

180 mg CaCO3

Test temperature:

control: 21.8-22.7 °C
100 mg/L: 21.7-23.1 °C

pH:

control: 7.5-7.9
100 mg a.i./L: 7.4-7.9

Dissolved oxygen:

control: 8.5-9.2 mg/L (6.2 mg/L after 29 hours)
100 mg/L: 8.4-9.2 mg/L (5.5 mg/L after 29 hours)
aeration was introduced after 29 hours, as the oxygen concentration tended to drop below the optimum level for testing with carp

Nominal and measured concentrations:

0.1, 1.0, 10 and 100 mg a.i./L
Analysis of the samples taken at the start and the end of the test showed that measured concentrations were in agreement with nominal (100 %).

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 litres
- Material, size, headspace, fill volume: all-glass, 7.7 L test solution
- Aeration: introduced after 29 hours
- No. of organisms per vessel: 3 fishes/concentration (range-finding test), 7 fishes/concentration (limit test)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):1
- Biomass loading rate: 0.48 fish/litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: adjusted ISO medium


OTHER TEST CONDITIONS
- Adjustment of pH: only before testing period
- Photoperiod: 16 h daily
- Light intensity: o information available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : lethality (24, 48, 72, 96 h)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 for range-finding test
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 0.1, 1.0, 10 mg/L

Reference substance (positive control):

yes

Remarks:

pentachlorophenol (PCP)

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

behaviour

Details on results:

- Behavioural abnormalities: no clinical effects were observed

Results with reference substance (positive control):

- Results with reference substance valid? yes, findings of LC50(96h) were within the range of historical data
- Mortality: yes
- LC50: 0.15 mg/L (95% c.i.: 0.10-0.22 mg/L)

Reported statistics and error estimates:

no statistics reported due to absence of effects in test

Sublethal observations / clinical signs:

Table 1. Incidence of mortality and total mortality during range-finding test

Concentration HAPS (mg a.i./L)	Initial number of fish	Cumulative mortality					Total mortality (%)
		4 h	24 h	48 h	72 h	96 h
0.1	3	0	0	0	0	0	0
1.0	3	0	0	0	0	0	0
10	3	0	0	0	0	0	0
100	3	0	0	0	0	0	0

 

Table 2. Incidence of mortality and total mortality during limit test

Concentration HAPS (mg a.i./L)	Initial number of fish	Cumulative mortality					Total mortality (%)
		3¼h	24 h	48 h	72 h	96 h
Control	7	0	0	0	0	0	0
100	7	0	0	0	0	0	0

 

 

Validity criteria fulfilled:

yes

Conclusions:

No visible effects observed in carp under test conditions up to highest test concentrations.

Executive summary:

Based on the results of a preceding range-finding test a final test was performed as a limit test. Seven carp per test group were exposed to a control and a HAPS concentration of 100 mg a.i./L. The total test period was 96 hours and a static test system was applied. Samples for analytical confirmation of actual exposure concentrations were taken at the start and the end of the test period.

Analysis of the samples taken at the start and the end of the limit test showed that measured concentrations were in agreement with nominal (100 %). The study met the acceptability criteria prescribed by the protocol and was considered valid.

HAPS induced no visible or lethal effects in carp at or below 100 mg a.i./L (NOEC). The 96h-LC50 exceeded 100 mg a.i./L (corrected for purity and based on analytically confirmed nominal concentrations).

Description of key information

In absence of visible effects in available study with fish key value is set to the maximal concentration used in the study for the further assessment. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L