Reaction mass of 2-(2-thienyl)ethyl benzenesulphonate and toluene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP, non guideline, published in peer reviewed literature, acceptable with restrictions.

Data source

Reference

Reference Type:

publication

Title:

The correlation of the toxicity to algae of hydrocarbons and halogenated hydrocarbons with their physical-chemical properties.

Author:

Hutchinson TC, Hellebust JA, Tam D, Mackay D, Mascarenhas RA, Shiu WY

Year:

1980

Bibliographic source:

Environ. Sci. Res. 16, 577-586.

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Two algal strains were exposed to C14 labelled toluene for 3 hours in a closed system. After 3 hours the amount of radiolabelled carbon taken up by the algal cells was measured and used to report the level of photosynthesis inhibition by toluene.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Toluene

EC Number:

203-625-9

EC Name:

Toluene

Cas Number:

108-88-3

Molecular formula:

C7H8

IUPAC Name:

toluene

Details on test material:

C14 labelled toluene

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

No analytical confirmation of test concentrations

Test solutions

Vehicle:

no

Details on test solutions:

Three test concentrations, negative control and dark controls included.

Test organisms

Test organisms (species):

other: Chlorella vulgaris and Chlamydomonas angulosa

Details on test organisms:

#260 Indiana collection and #680 Indiana collection respectively. Three to four day exponential phase cells were used at a concentration of 20E4 cells.ml-1 for Chlorella vulgaris and 5E4 cells.ml-1 for Chlamydomonas angulosa.

Study design

Test type:

other: static, closed

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

None

Test conditions

Test temperature:

19 °C

pH:

6.5

Salinity:

not applicable

Nominal and measured concentrations:

20, 50 and 100% saturated solution

Details on test conditions:

12L:12D, dark controls. After three hours the cells were washed to remove surface radioactivity and the amount of radioactivity taken up by the algal cells determined on dried Millipore filters with an end window gas flow counter.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

134 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: photosynthesis rate

Remarks on result:

other: Chlamydomonas angulosa

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

207 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: photosynthesis rate

Remarks on result:

other: Chlorella vulgaris

Details on results:

Uptake of 14CO2 used as an indication of photosynthesis rate. EC50 equal 134 mg/L and 207 mg/L respectively.

Results with reference substance (positive control):

not applicable

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

The 3 hour EC50 for reduced photosynthesis rate is 134mg/l.