Registration Dossier
Registration Dossier
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EC number: 217-121-1 | CAS number: 1745-89-7
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Determination of the acute toxicity of TK 11907 to Daphnia magna.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.64 mg/L
Additional information
The study was performed to assess the acute toxicity of the test item toDaphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphniasp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.
Methods:….
Information provided by the Sponsor indicated the test item was practically insoluble in water.
Preliminary solubility work conducted indicated that it was not possible to obtain a testable solution of the test item using traditional methods of preparation e.g. ultrasonication and high shear mixing.
A preliminary media preparation trial indicated that a dissolved test item concentration of approximately 7.7 mg/L was obtained from a saturated solution method of preparation indicating this to be the limit of water solubility of this item under test conditions.
Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at nominal concentrations of 1.0, 1.8, 3.2, 5.6 and 10% v/v saturated solution for 48 hours at a temperature of approximately 21°C under static test conditions. The test item solutions were prepared by stirring an excess (50mg/L) of test item in test water using a propeller stirrer at approximately 1500 rpm for 24 hours. After the stirring period any undissolved test item was removed by filtration (0.2 µm Sartorius Sartopore filter, first approximate 2 liters discarded in order to pre-condition the filter). This saturated solution was then further diluted as necessary, to provide the remaining test concentrations. Immobilization and any adverse reactions to exposure were recorded after 24 and 48 hours.
Results:
Analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.21 to 3.0 mg/L. There was no significant change in the measured concentrations at 48 hours and so the results are based on 0-Hour measured test concentrations only.
Exposure ofDaphnia magnato the test item gave the following results based on the 0-Hour measured test concentrations:
Time Point (Hours) |
EC50 |
95% Confidence Limits (mg/L) |
No Observed Effect Concentration (NOEC) (mg/L) |
Lowest Observed Effect Concentration (LOEC) (mg/L) |
||
48 |
0.64 |
0.59 |
- |
0.69 |
0.41 |
0.87 |
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