Vanadium trichloride oxide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: study not GLP but in which all parameters described are comparable to guideline methods for testing acute toxicity to fish

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Toxicity of Vanadium to Different Freshwater Organisms.

Author:

Beusen J.M. and Neven B.

Year:

1987

Bibliographic source:

Bulletin of Environmental Contamination and Toxicology, 39, 194-201.

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Remarks:

even if not all data are indicated

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

sodium metavanadate NaVO3

IUPAC Name:

sodium metavanadate NaVO3

Test material form:

not specified

Details on test material:

purity > 98%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

not specified

Details on test solutions:

Dilution water used for stock cultures and tests is synthetic hard water:
composition (mg/L deionized water):
100 mg NaHCO3,
20 mg KHCO3,
180 mg MgSO4.7H2O
200 mg CaCl2.2H2O

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Brachydanio rerio are obtained from a commercial supply house.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

no data

Test temperature:

23°C +/-2°C

pH:

7.7 to 8.5

Dissolved oxygen:

above 6 mg/L

Salinity:

not given

Nominal and measured concentrations:

Not given, but the measured vanadium concentrations were within 10% of the initial amount of the metal added to each test vessel.
A control plus at least 5 concentrations were selected on a basis of a logarithmic expansion.

Details on test conditions:

Before testing, fishes from a single stock of similar lenght and age are adapted to laboratory conditions in 200 L tanks filled with hard water for at least 12 days. They were fed twice daily, ceasing 24h before initiation of the test.
photoperiod: 14h daylight/ 10h darkness

TEST SYSTEM
- Test vessel: 1L beakers filled with 1L of test solution
- fishes are transferred to new test medium after 48 and 96h
- Aeration: gentle bubble aeration
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): duplicate

Before testing, fishes from a single stock of similar lenght and age are adapted to laboratory conditions in 200 L tanks filled with hard water for at least 12 days. They were fed twice daily, ceasing 24h before initaition of the test.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 14h daylight/ 10h darkness

EFFECT PARAMETERS MEASURED: mortalities, at 24h intervals

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

element

Basis for effect:

mortality (fish)

Remarks on result:

other: geometric mean of 3 data

Details on results:

the 96h LC50 data are as follows:
2.9 (CI: 2.1- 3.4)
4.1 (CI: 2.6- 6.4)
5.3 (CI: 3.4- 8.4)

Applicant's summary and conclusion

Executive summary:

Zebrafish; Danio rerio were exposed to a control and at least 5 measured vanadium concentrations during 96h under static conditions and in a way similar to the guidelines. The 96h-LC50 for mortality was determined to be 4 mg V/L (geometric mean of 3 replicates).