Oxalonitrile

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1960

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Four male albino rabbits whose weights averaged 4.5 pounds were closely clipped of their fur, restrained in a wooden stock, and placed in the chamber. An especially designed door, through which the rabbits heads extended into glass jarsmaintained under positive air pressure, prevented the rabbits from breathing cyanogen while their bodies were being exposed and also made observation of the heads of the rabbits possible. The bodies of the rabbits were exposed to ethanedinitrile concentration of 10,000 ppm for a period of 8 hours.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Oxalonitrile

EC Number:

207-306-5

EC Name:

Oxalonitrile

Cas Number:

460-19-5

Molecular formula:

C2N2

IUPAC Name:

carbononitridic cyanide

Test material form:

gas

Specific details on test material used for the study:

ethanedinitrile 99.5 %

Test animals

Species:

rabbit

Strain:

not specified

Remarks:

albino rabbit

Sex:

male

Details on test animals or test system and environmental conditions:

Four male albino rabbits whose weights averaged 4.5 pounds were closely clipped of their fur, restrained in a wooden stock, and placed in the chamber. An especially designed door, through which the rabbits heads extended into glass jarsmaintained under positive air pressure, prevented the rabbits from breathing cyanogen while their bodies were being exposed and also made observation of the heads of the rabbits possible. The bodies of the rabbits were exposed to ethanedinitrile concentration of 10,000 ppm for a period of 8 hours.

Administration / exposure

Type of coverage:

open

Vehicle:

other: air

Duration of exposure:

8 hours

Doses:

10 000 ppm (ca 21 g/m3)

No. of animals per sex per dose:

4

Control animals:

yes

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

other: no effect

Remarks:

8-hour exposure

Effect level:

10 000 other: ppm

Based on:

act. ingr.

Remarks on result:

no indication of skin irritation up to the relevant limit dose level

Mortality:

no mortality observed

Clinical signs:

other: no effects observed

Gross pathology:

no effects observed

Other findings:

no effects observed

Applicant's summary and conclusion

Interpretation of results:

other: CLP criteria not met

Conclusions:

Dermal exposure of rabbits to ethanedinitrile gas in an acute toxicity study indicates that there is no intake of ethanedinitrile through skin. Dermal exposure of rabbits to 10,000 ppm (~21 g/m3) of ethanedinitrile for eight hours did not result in toxicity towards rabbits, or any clinical observations or macroscopic effects.

Executive summary:

Materials and methods

Four male albino rabbits whose weights averaged 4.5 pounds were closely clipped of their fur, restrained in a wooden stock, and placed in the chamber. An especially designed door, through which the rabbits heads extended into glass jars maintained under positive air pressure, prevented the rabbits from breathing cyanogen while their bodies were being exposed and also made observation of the heads of the rabbits possible. The bodies of the rabbits were exposed to ethanedinitrile concentration of 10,000 ppm for a period of 8 hours.

Conclusion

Dermal exposure of rabbits to ethanedinitrile gas in an acute toxicity study indicates that there is no intake of ethanedinitrile through skin. Dermal espousure of rabbits to 10,000 ppm (~21 g/m³) of ethanedinitrile for eight hours did not result in toxicity towards rabbits, or any clinical observations or macroscopic effects .