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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
1989-10-18 to 1989-11-11
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
No chemical analysis due to poor solubility
Justification for type of information:
See attached read across document
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1984
Deviations:
yes
Remarks:
The study was performed without analytics.
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
1984
Deviations:
yes
Remarks:
The study was performed without analytics.
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1000 mg of the test item to 1 L of diluting water and subsequent stirring for 24 hours at room temperature to reach an equilibrium concentration of the test substance in the test substance in the test medium
- Eluate: diluting water
- Controls: yes
Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM
- Common name: Poecilia reticulata (guppy)
- Source: IOB (Instituut voor Onderzoek van Bestrijdingsmiddelen), Wageningen, The Netherlands
- Age at study initiation: > 4 weeks
- Length at study initiation: approximately 2 cm


Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
13°dH
Test temperature:
22 - 26 °C
pH:
7.5 to 8.2
Dissolved oxygen:
5.9 to 7.8 mg/L
Nominal and measured concentrations:
- nominal concentration: 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 2 L covered glass aquaria which contained 1 L of the test medium
- Type (delete if not applicable): closed
- No. of organisms per vessel: 10 fishs in the control vessel and in the test vessel, whereas the duplicate test vessel contained 11 fishs
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.6 g biomas/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: a synthetic water, Dutch Standard Water according NPR 6507 (1980)
- Conductivity: less than 5 µS/cm
- Intervals of water quality measurement: Measurements of oxygen concentration were carried out on days 2 and 4, pH-measurements on days 0, 2 and 4. If a high mortality was observed on the other days, the oxygen concentrations and pH were measured then also.

OTHER TEST CONDITIONS
- Photoperiod: 12 hours per day

EFFECT PARAMETERS MEASURED: The surviving fish were counted daily and dead fish removed immediately upon observation.

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1, 10, 1000 mg/L
- Results used to determine the conditions for the definitive study: No mortality was observed.
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The fish survived in a saturated solution containing 1 g the test item /L for 96 hours. Binormal theory dictates that when 10 fish are used with 0 % mortality, there is a 99.9 % confidence that the LC50 (96 h) is greater than 1000 mg/L. However, given that during the test flakes of the test item remained visible at the bottom of the vessel, the LC50 should be handled with care.
No effects such as deviations in the behavior or appearance of the fish were not observed.
Validity criteria fulfilled:
yes
Conclusions:
The LC50 (96 h) of ditetradecyl peroxydicarbonate is greater than 1000 mg/L.
Executive summary:

Ditetradecyl peroxydicarbonate was tested in an acute toxicity test with Poecilia reticulata (guppy) under semi-static conditions in accordance with EU method C.1 and OECD guideline No. 203. The test was performed in duplicate. The fish survived for four days in a saturated solution which contained 1000 mg of the test substance/L. Binormal theory dicates that when 10 fish are used showing 0 % mortality, there is a 99.9 % confidence that the LC50 (96 h) is greater than 1000 mg/L. However, given that during the test flakes of the test substance remained visible at the bottom of the vessel, the LC50 value should be handled with care. No deviations in the behaviour or appearance of the fish were observed.

Description of key information

There is one data point available for Dicetylperoxydicarbonate and two data points available for the closely related material Dimyristiylperoxydicarbonate. For additional information see the accompanying readacross document. Due to the physical chemical properties of these material being similar and the toxicity to fish embryos for both materials both being above the solubility limits of both materials. It is considered justified to read across to the existing adult fish endpoint for Dimyristiylperoxydicarbonate. This study has therfore been highlighted as the key information for this endpoint. The LC50 was not reached in any of the three available data points. Due to greater than values (>) not being acceptable in the fields below the LC50 has been entered as 1000 mg/L

Key value for chemical safety assessment

LC50 for freshwater fish:
1 000 mg/L

Additional information

In addition, algae and daphnia endpoints (4x studies) for Dicetylperoxydicarbonate and Dimyristiylperoxydicarbonate have all showed no effects at the limit of solubility (EC50 > water solubility). In combination with the (2x) fish embryo toxicity endpoints also showing (EC50 > water solubility for fish species) and the similar physical/chemical properties and almost identical chemical structure. It is considered justified to use the existing acute adult fish test data to fulfil this endpoint and avoid further animal testing.