6-methyl-1,3,5-triazine-2,4-diyldiamine

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July - October 1972

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No guideline for the testing methode is mentioned in the report. Nevertheless the test result seems to be reliable as the observation period is also stated to be 14 days and the testing procedure as well as the choice of doses seems to be reasonable.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,4-Diamino-6-methyl-1,3,5-triazine

IUPAC Name:

2,4-Diamino-6-methyl-1,3,5-triazine

Constituent 2

Reference substance name:

6-methyl-1,3,5-triazine-2,4-diyldiamine

EC Number:

208-796-3

EC Name:

6-methyl-1,3,5-triazine-2,4-diyldiamine

Cas Number:

542-02-9

Molecular formula:

C4H7N5

IUPAC Name:

6-methyl-1,3,5-triazine-2,4-diamine

Details on test material:

- Name of test material (as cited in study report): Acetoguanamine
- Appearance/physical state/colour: white powder

Test animals

Species:

rat

Strain:

other: Wistar derived

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: colony of TNO institute
- Weight at study initiation: 150 to 260 g (males) and 108 to 208 g (females)
- Fasting period before study: 16 hours
- Stock diet and tap water: ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Details on oral exposure:

VEHICLE
- Concentration in vehicle: a 20 % (w/v) suspension of Acetoguanamine in a 5 % aqueous solution of carboxy methyl cellulose

MAXIMUM DOSE VOLUME APPLIED:
15 ml of the 20 % suspension of Acetoguanamine in a 5 % aqueous solution of carboxy methyl cellulose for each kg of body weight.

Doses:

10, 11, 12, 13, 14 and 15 ml of the 20 % suspension of Acetoguanamine in a 5 % aqueous solution of carboxy methyl cellulose for each kg of body weight.

No. of animals per sex per dose:

five

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes

Statistics:

no statistics reported

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LD50

Effect level:

2.74 other: g/kg

95% CL:

2.57 - 2.92

Mortality:

Some of the animals died within three hours after the treatment with Acetoguanamine.
Deaths occurred up to two days after dosing.

Clinical signs:

other: One hour after the treatment all animals became sluggish and showed severe diarrhoea. But the survivors recovered rapidly and looked quite healthy again at the end of the first week.

Gross pathology:

No abnormalities were seen in these animals at autopsy.

Other findings:

no data

Any other information on results incl. tables

TABEL 1: The mortality in the test animals of the various dose groups after the treatment with Acetoguanamine.

dose		mortality
ml 20 % solution/kg	g test substance/kg	number		%
		males	females
10	2.0	0/5	0/5	0
11	2.2	0/5	2/5	20
12	2.4	1/5	0/5	10
13	2.6	0/5	2/5	20
14	2.8	2/5	4/5	60
15	3.0	3/5	5/5	80

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 value of Acetoguanamine was calculated to be 2.74 g/kg body weight with 2.92 and 2.57 as 95 % confidence limits. Because of this reason Acetoguanamine is not classified as acute oral toxic.

Executive summary:

An acute oral toxicity test with Acetoguanamine was performed. No guideline for the testing methode is mentioned in the report. Nevertheless the test result seems to be reliable as the observation period is also stated to be 14 days and the testing procedure as well as the choice of doses seems to be reasonable.

On the basis of some preliminary observations the following doses were given as as one single dose to groups of five male and five female rats: 2.0, 2.2, 2.4, 2.6, 2.8, 3.0 g test substance / kg bw.

The animals were observed for a 14 -day period. Then the survivors were killed and examined grossly. With the obtained study results the LD50 of Acetoguanamine was calculated to be 2.74 g/kg body weight. Because of this reason Acetoguanamine is not classified as acute oral toxic.