L-Proline, 4-(phenylthio)-, (4S)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(2S,4S)-4-(phenylsulfanyl)pyrrolidine-2-carboxylic acid

EC Number:

617-252-8

Cas Number:

81653-77-2

Molecular formula:

C11H13NO2S

IUPAC Name:

(2S,4S)-4-(phenylsulfanyl)pyrrolidine-2-carboxylic acid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

Five different concentrations in a geometric series, namely 10.0, 17.8, 31.6, 56.2 and 100.0 mg/L.
The test was performed in 50 mi glass beakers filled with 40 mi of test medium.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The study was performed on thè freshwater crustacean Daphnia magna Straus.
At the start of the test, organisms used in the test were 6-24 hours old and were not first brood progeny.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

water hardness = 250 mg/l

Test temperature:

The incubation temperature was continuously monitored during the course of the study by the means of a data logger. The measured values ranged between 20.6 and 22.5 °C during the test period, only slightly upper than the OECD Guideline recommended range (20.0 ± 2.0 °C).

pH:

The pH of the test medium after preparation was in the range 7.50 – 7.59, the pH of control was 7.36.

Nominal and measured concentrations:

10.0, 17.8, 31.6, 56.2 and 100.0 mg/L - nominal

Details on test conditions:

Due the light sensitivity of test item, the test was performed in dark conditions.
A negative control with reconstituted water only was also tested.
For each test concentration and for the control, 20 daphnids were used divided into four replicates of five daphnids each. The daphnids were randomly distributed to thè test vessels
at initiation of thè test. The daphnid s loading rate was more than one per 2 mi of test
solution. The test vessels were replaced in thè dark place to avoid thè penetration of thè light
in thè test vessels.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

IC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Remarks on result:

other:

Remarks:

95% Confidence range 98.6-101.3

Effect concentrations 2

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

56.2 mg/L

Nominal / measured:

nominal

Effect concentrations 3

Duration:

24 h

Dose descriptor:

LOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Effect concentrations 4

Duration:

48 h

Dose descriptor:

IC50

Effect conc.:

70 mg/L

Nominal / measured:

nominal

Remarks on result:

other:

Remarks:

95% confidence range 55.7-85.3

Effect concentrations 5

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

17.8 mg/L

Nominal / measured:

nominal

Effect concentrations 6

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

31.6 mg/L

Nominal / measured:

nominal

Details on results:

No remarkabie obsen/ations were made conceming thè appearance of the test medium. The test medium was a clear solution throughout the whole test duration.
In the negative control, 0% immobilization was observed during thè test period at each observation time.

Results with reference substance (positive control):

24 h IC50 (mg/L) = 1.22
Confidence interval (95%, mg/L) = 0.75-1.77
Reference data (UNI EN ISO 6341/99) = 0.6-2.1

Reported statistics and error estimates:

The results were analyzed for significant differences from control with thè Fisher s Exact test (a = 0.1) by thè means of CETIS elaboration software V1.026D.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations:
24 h NOEC: 56.2 mg/L
24 h LOEC: 100.0 mg/L
24h IC50: 100.0 mg/L
48 h NOEC: 17.8 mg/L
48 h LOEC: 31.6 mg/L
48 h IC50: 70.0 mg/L

Executive summary:

The acute toxicity of test item was tested on Daphnia magna under static conditions, according to the OECD guideline 202 (2004).

The NOEC, LOEC and IC50 values after 24 and 48 h, caiculated based on nominal concentrations were as follows:

24 h NOEC: 56.2 mg/L

24 h LOEC: 100.0 mg/L

24h IC50: 100.0 mg/L

48 h NOEC: 17.8 mg/L

48 h LOEC: 31.6 mg/L

48 h IC50: 70.0 mg/L