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EC number: 201-137-0 | CAS number: 78-73-9
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
No toxicity observed even at highest concentration level tested.
RA_CAS 67-48-1_Daphnia magna_EU Method C.2: EC0(48h): 125 mg/L, EC50(48h): 500 mg/L, EC100(48h) >= 500 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 500 mg/L
Additional information
Short-term toxicity to aquatic invertebrates was not investigated with the target substance Choline hydrogen carbonate. A valid experimental result is available for the reliable read-across substance Choline chloride (CAS 67-48-1). Both substances belong to the group of quaternary amine salts (more specific: choline salts, i.e. (2-hydroxyethyl)trimethylammonium salts), which will dissociate immediately in aqueous environment. Choline and its metabolites are important for different functions in the body of animate beings and thus a naturally occurring compound. Thus, the substance characteristics of both substances are comparable. For the detailed justification of this procedure, please refer to the separate read-across statement by Chemservice S.A. (2013).
The acute toxicity of Choline chloride towards the aquatic invertebrate Daphnia magna was investigated according to EU Method C.2. (BASF AG, 1988). Test concentrations were chosen as followed: 0 (control), 31.25, 62.50, 125, 250 and 500 mg/L. Four replicates of each concentration were included. The mobility and the corresponding EC0, EC50 and EC100 values were determined after 3, 6, 24 and 48 hours. The highest concentration tested at which an effect =< 10 % occurred was referred to as EC0. Physico-chemical measurements (temperature, pH and dissolved oxygen content) were measured at the beginning and termination of the experiment. At the start, the temperature was recorded as 18.75 °C and the pH 7.8. The exposure duration was 48 h. Results were reported as: EC0(48h): 125 mg/L, EC50(48h) >= 500 mg/L and EC100(48h) >= 500 mg/L. No analytical verification and no statistical methods were implemented in the experiment.
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