Reaction mass of (3R)-3-[(1R)-4-methyl-3-cyclohexen-1-yl]-1-butanol and (3S)-3-[(1R)-4-methyl-3-cyclohexen-1-yl]-1-butanol

Short-term toxicity to aquatic invertebrates

Description of key information

EC50 (invertebrates)= 7.1 mg/l (95% CL: 5.0-10 mg/l), 48 hour, freshwater, OECD 202, Parr 2013
NOEC (invertebrates)= 5.0 mg/l, 48 hour, freshwater, OECD 202, Parr 2013
LOEC (invertebrates)= 10 mg/l, 48 hour, freshwater, OECD 202, Parr 2013

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

7.1 mg/L

Additional information

The acute toxicity to Daphnia magna was carried out according to OECD 202 Daphnia sp., Acute Immobilisation Test and EU Method C.2 guidelines. A pre-study media preparation trial indicated that the use of a saturated solution method of preparation was most appropriate for this test item. Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 daphnids) were exposed to an aqueous solution of the test item at nominal concentrations of 1.0, 1.8, 3.2, 5.6 and 10% v/v saturated solution for 48 hours at a temperature of 20 °C to 21 °C under static test conditions. The test item solution was prepared by stirring an excess of test item in test medium using a propeller stirrer for 24 hours. After the stirring period any undissolved test item was removed by filtration to produce a 100% v/v saturated solution of the test item from which dilutions were made to give the test series. The number of immobilized Daphnia were recorded after 24 and 48 hours. Analysis of the 3.2, 5.6 and 10% v/v saturated solution test concentrations at 0 hours showed measured concentrations of 6.6, 12 and 24 mg/L. This declined at 48 hours to 3.8, 8.9 and 19 mg/L respectively. Given this decline in measured test concentrations it was considered justifiable to base the results on the geometric mean measured test concentrations. The 48h-EC50 was 7.1 mg/l (95% CL: 5.0 – 10 mg/l) based on GMM concentration.