Coconut oil, reaction products with polyethylene glycol and trimethylolpropane

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08/09-1996

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)

Principles of method if other than guideline:

Toxizitaetstest fuer Regenwuermer in künstlichem Boden nach 88/302 EWG

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of coconut oil with polyethyleneglycol and 2-ethyl-2-(hydroxymethyl)propane-1,3-diol

EC Number:

640-964-5

Cas Number:

218451-68-4

Molecular formula:

not applicable because UVCB

IUPAC Name:

Reaction products of coconut oil with polyethyleneglycol and 2-ethyl-2-(hydroxymethyl)propane-1,3-diol

Details on test material:

- Name of test material (as cited in study report): Marlowet 4753
- Physical state: liquid, cristalline
- Stability under test conditions: > 1 year

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil: The test substance was mixed with silica sand and afterwards added to soil substrate in a plastic bag. Water was added to a theoretical water content of approx. 40% and the substrate was thoroughly mixed
pH = 6

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworm
- Source: Eisenia GmbH&Co. Produktions- und Vertrieb KG, Kapellenstraße 25, Wiesbaden, Germany
- Age at test initiation: at least 2 months
- Weight at test initiation: average 350 mg (control), average 354 mg (test substance)

ACCLIMATION
- Acclimation period: not mentioned

Study design

Study type:

laboratory study

Substrate type:

other: peat and dried horse dung

Limit test:

yes

Total exposure duration:

14 d

Post exposure observation period:

no

Test conditions

Test temperature:

20 +- 2 °C

pH:

6

Moisture:

40 %

Details on test conditions:

TEST SYSTEM
- Test container (material, size): Makrolon cages with perforated plexiglass covers, size not mentioned
- Amount of soil or substrate: not mentioned
- No. of organisms per container (treatment): 10

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): mixture of peat and horse dung
- Organic carbon (%): not mentioned
- Maximum water holding capacity (in % dry weigth): 40 %
- CEC: not mentioned
- Pretreatment of soil: not mentioned
- Storage (condition, duration): fresh prepared
- Stability and homogeneity of test material in the medium: not mentioned

OTHER TEST CONDITIONS
- Photoperiod: 24 h
- Light intensity: approx. 400 lux

VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable, limit test
- Range finding study: not performed

Nominal and measured concentrations:

not measured; nominal concentration 1000 mg/kg dry soil

Reference substance (positive control):

yes

Remarks:

chloracetamide, historical data

Results and discussion

Effect concentrations

Key result

Duration:

14 d

Dose descriptor:

LC10

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality at end of exposure period: 1
- Total mass of adults at beginning of test: 14 g (test substance), 14.16 g (control), 40 adults each
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: not mentioned
- No. of offspring produced: not mentioned
- No. of unhatched cocoons: not mentioned
- Morphological abnormalities: not mentioned
- Behavioural abnormalities: not mentioned
- Other biological observations: not mentioned

Results with reference substance (positive control):

RESULTS: TEST WITH REFERENCE SUBSTANCE (historical data, from 1996-03-13 to 1996-03-27)
- Concentrations: 0, 10, 30 and 100 mg 2-chloroacetamide/kg dry soil
- Results (mortality): 0, 10, 30 mg/kg: 0/20 (0%) after 7 and 14 days, respectively; 100 mg/kg: 20/20 (100%) after 7 and 14 days, respectively.

Any other information on results incl. tables

RESULTS: EXPOSED

- Nominal concentrations: 1000 mg/kg dry soil

- Effect data (Mortality): 0/40 (0%) after 7 days; 1/40 (2.5%) after 14 days

RESULTS: CONTROL

- Effect data (Mortality): 0/40 (0%) after 7 and 14 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

In the toxicity limit test with Eisenia foetida foetida the test substance Marlowet 4753 (Coconut oil, reaction products with polyethylene glycol and trimethylolpropane) showed at a concentration of 1000 mg/kg (<= 10 %) no mortality after 7 and 14 days.